| # Anti-CoV Ab Panel x CoV2-RBD Mutagenesis (YM_0005) | |
| ## Overview | |
| YM_0005 is a Covid receptor-binding domain (RBD) single-site mutagenesis (SSM) library against a panel of 33 ScFvs. | |
| ## Experimental details | |
| We studied the effects of a panel of ScFvs against COVID RBD. In this dataset, our panel of ScFvs are tested in 2 orientations: LH for light-heavy chains and HL heavy-light chains. We explore the local landscape of the RBD in tandem with Covid for epitope mapping by mutating each position. We include a control of ACE2, as the protein of most therapeutic antibodies disrupt. | |
| This dataset includes 62 unique scFvs and 2431 unique RBD sequences. | |
| A more extensive methods section can be found in our publication [here](https://academic.oup.com/abt/article/5/2/130/6584706). | |
| ## Misc dataset details | |
| We define the following binders: | |
| ### A-library (scFvs) | |
| - Binder descriptor. HL / LH denotes orientation of the scFv as expressed on the yeast surace. | |
| ### Alpha-library | |
| - Any `matalpha_description` that contains the term "WT" can be assumed as a replicate of the WT target. Other sequences will be indicated by their original residue, position of mutation, and mutated residue (i.e. S23A is Ser -> Ala mutation in position 23). | |