data/YM_0005/README.md

Anti-CoV Ab Panel x CoV2-RBD Mutagenesis (YM_0005)

Overview

YM_0005 is a Covid receptor-binding domain (RBD) single-site mutagenesis (SSM) library against a panel of 33 ScFvs.

Experimental details

We studied the effects of a panel of ScFvs against COVID RBD. In this dataset, our panel of ScFvs are tested in 2 orientations: LH for light-heavy chains and HL heavy-light chains. We explore the local landscape of the RBD in tandem with Covid for epitope mapping by mutating each position. We include a control of ACE2, as the protein of most therapeutic antibodies disrupt.

This dataset includes 62 unique scFvs and 2431 unique RBD sequences.

A more extensive methods section can be found in our publication here.

Misc dataset details

We define the following binders:

A-library (scFvs)

• Binder descriptor. HL / LH denotes orientation of the scFv as expressed on the yeast surace.

Alpha-library

• Any matalpha_description that contains the term "WT" can be assumed as a replicate of the WT target. Other sequences will be indicated by their original residue, position of mutation, and mutated residue (i.e. S23A is Ser -> Ala mutation in position 23).