I am a class 12 student and would like to know how to extract DNA from an egg in a school level BIO-tech LAB?
553567
How can you probably have a school lab with that kinda facility. DNA extraction from an egg requires much more sophisticated lab.
507
Put the egg in the middle of a 1 foot square styrene block. \nFit the egg with a parachute. \nput it in strong bottle filled with water before dropping it.
1142067
DNA = deriboneucleic acid\nRNA = riboneuclic acid\nRNA is present in each human cell in the lipoprotein (outside the cell) part. \nDNA is present in the neucleus of the cell. to be more specific, in the chromosomes inside the neucleus of the cell. so, it is only the DNA that carries the genetic property to the offsprings.\n\nas for your project, you can surely include it as a chapter.however make it sure that it is not too lengthy to dominate the main subject.\nyou can also include a chapter related to "clones and cloning". that could help you in showing it more clearly that its only the DNA that helps to transfer the genetic property to the young ones.\nif you need any further help in your project, you can contact me at \n\nmangeshome1982@yahoo.co.in\n\ngood luck.
384036
Heres a cool one: http://gslc.genetics.utah.edu/units/activities/extraction/
80422
Sorry, your question has more to be desired grammatically. Are you looking for the answers to the AP Bio Lab manual?\nhttp://members.cox.net/ccjjr59/lab1.html\nJust change the number of the lab before ".html" in the address for the lab you're at.\n\nIf this isn't what you need, repost with a question that is more clear.
62320
well, hope these help:\n\nhttp://www.bbc.co.uk/dna/h2g2/A388217\n\nhttp://www.chemistrysurvival.com/
865983
Your best bet would be to contact your local community college, or university, and ask to speak to whomever is in charge of the microbiology dept. and explain what your intentions are and they can let you know if you can indeed "use" their lab.  I have to say, however, if your actual project involves bacteria it is going to be a bit of a problem "removing your project" from that lab. You can chart and record your findings and perhaps do a photo pictorial outline, but you will not be allowed to remove any cultures or plates that have grown. (The labs are all the same with this policy/procedure as it falls under watch of the regulatory divisions in the government.  Think about it...they don't want positive growth plates to possibly contaminate outside of the research area...regardless of the specimen.)  Hope this helps and good luck with your project.
32578
This is a fantastic question. There is an old joke that DNA extraction is like playing with test tubes of water. That's because DNA floating around in water is invisible, and looks like the researcher is just pouring clear water from one container to another.\n\nThere are several methods, called protocols, for isolating DNA from plants. A google search on plant dna extraction will give you a boatload of protocols. Most require exotic chemicals and lab equipment. \n\nSee the complicated lab method link below.\n\nBut you can extract DNA from plants in your kitchen using a blender, detergent, meat tenderizer, and rubbing alcohol! \n\nIn the case of Cleome, I would use about half a cup of the seed pods. Best of luck!
796672
OK, draw yourself up a nice big checkerboard with 16 squares! Down the left hand side, write beside each square the 4 possible gametes produced by 1 parent - for parents with the genotypes you have given above, the gametes produced are: AB, Ab, aB, ab - this is because each egg or sperm produced will have only half the number of genes of the parent (so ONE copy instead of 2) so that when the egg and sperm meet, they will have 2 copies of each and not more!!! So the gametes will have ONE copy of the 'A' gene (which may be either the dominant 'A' allele or recessive 'a' allele - half the gametes will get one type of allele and half the other) and the same goes for the 'B' gene, to produce the possible gamete types above. Then, along the top of your little checkerboard diagram, write above each of the 4 squares the 4 possible gamete types for the SECOND parent - which in your case, are the same as the first!\n\nNow - you want to fuse your eggs and sperm to produce offspring! Do this by combining the gamete genotype of 1 parent with that of the other in the box where they meet up - eg if your very top left-hand square has the genotype 'AB' above it, and 'AB' to the left of it, then the genotype of the offspring produced will be AABB - homozygous for both! And the square 2 down from the top, in most left column, might be combining the gametes 'Ab' with AB - producing offspring AABb, which is homozygous for gene 'A' but not for gene 'B'.... and so on and so forth for the rest of the squares!\nI WOULD RECOMMEND DRAWING UP THIS SQUARE TO SHOW YOUR WORKING - YOUR TEACHER WILL BE WANTING TO SEE THAT!\ndouble homozygotes are AABB, AAbb, aaBB, or aabb...
709571
try this:  Stand on a lawn and throw an egg up into the air .. Just chuck it like 40 feet up and watch what happens when it lands on the lawn.  \n  Don't throw it on concrete and try to make sure it doesn't hit any rocks. \n  When I was in eigth grade and my science teacher told me to try this i was absolutly flabbergasted when I saw the results.  I ended out making 75$ from my family when I bet them I could throw an egg over the house and it wouldn't break when it landed on the other side.
1081930
Yes you can all you need to do is go to your teacher references and look it up if they do not have it then go to the library and ask if the have biology cd that covers all the topics.
554481
Can I have it? I love to collect unique specimens. I'll keep it in a jar on my desk at school and you can be our "class brain." *shrugs* Hey..it's one way to be the teacher's pet. :D
196815
tie a piece of string round the egg, and then measure the string
104426
DNA is a good source of evidence for forensic experts, for doctors to identify cancer, recently used for SARS detection, to name a few .\n\nThere are techniques which are used to detect the presence of a specific sequence in a given DNA sample. PCR  or Polymerase Chain Reaction is one of them. here we amplyfy a specific sequence of DNA of interest. if the biological sample contains that DNA sequence then its amplified and can be visualized on a electrophoretic gel. DNA being negativey charged runs on an agorose gel under electric current towards the positive electrode and gets separated based on the size...slower the movement the bulkier the fragment. \n\nforensic experts use this to match DNA samples from site of crime to the suspect. this can also be used in case of paternity disputes, to detect the predisposition of a person to cancer etc etc.\n\nthere are many more techniques which you can search
319169
You will not be able to do it for your class.  It takes a lot of time, skill, boats, oysters, and luck!\n\nSee for example:\n\nhttp://www.spc.int/coastfish/News/POIB/12/POIB12.pdf\n\nWhen I visitted Broome, in northern Western Australia, I saw how it is done.  It involves a skilled worker doing surgery on the living oyster, to place a "seed" into its matle.  Then, the oyster is returned to the sea, to recover, if it can, and to slowly cover the seed with extra nacre.  Then, it is harvested again; this time, the oyster is killed.\n\nYou need baby oysters, called sprat.  You need seed pearls.  You need to learn the technique, handed down from Mr Mikimoto of Japan.\n\nIt is not suitable as a practical exercise for schoolkids.
1154135
DNA Technology\nRecombinant DNA technology refers to the techniques used to transplant genes from one living source into another where it will be expressed.\n \nHow Does it work?:\nThe science of DNA technology includes the use of special enzymes called restriction enzymes, DNA vectors, and the host organisms. We will take a look at each of these groups in the following section.\n \nRestriction Enzymes:\nThese special enzymes were discovered in the late 1960's as naturally occurring agents in bacteria. They protect the bacterium against foreign DNA from other organisms. Invading DNA is cut into pieces and made inoperable. This process is called restriction. As with any enzyme, these are specific in the job they do. Many of them only recognize short, specific nucleotide sequences( recognition sequences) and cut at specific points within those sequences. Bacteria protect their own DNA by a process called methylation. During this process methyl groups are added to the nucleotides within the recognition sequence. There are several hundred restriction enzymes and about 100 different recognition sequences.\nRecognition Sequences: are symmetric in that the same sequence of 4 to 8 nucleotides is found on both strands, but run in opposite directions. The restriction enzymes usually cut the phosphodiester bonds of both strands in a staggered manner. The result being both ends have a single stranded area called the sticky ends. It is within this space that the new piece of DNA is added, attaching to the sticky ends. \n \nThese unions are temporary until the enzyme DNA ligase is added to catalyze the formation of the phosphodiester bonds.\nVectors: are used as carriers for moving DNA from test tubes into cells. Bacterial plasmids and viruses are the most widely used vectors in DNA transfer. Bacterial cells can pick up the DNA through the process of transformation. Lambda phages are used by eliminating the middle of its liner genome and adding the foreign DNA in the created space. The phage is then introduced into the bacterial cell where it replicated itself via the lysogenic cycle.\nHost Organisms: Bacteria are usually used as hosts in genetic engineering. There are several reasons why they are chosen. 1. Bacterial DNA can be easily isolated and reintroduced into bacterial cells. 2. Bacterial cultures grow quickly. Some disadvantages surface as well: 1. Bacteria, being prokaryotic, may not be able to use the information in eukaryotic genes. 2. Bacterial cells cannot make the the necessary changes in transcription to produce some eukaryotic proteins. Eukaryotic cells can also be used as hosts. Yeast cells and some plant and animal cells can be a host for foreign DNA, but it is often difficult to get such cells to take up engineered DNA.\nSteps for using Bacteria and Plasmids to Clone Genes:\n1. Isolation of two kinds of DNA.\n2. Treatment of plasmid and foreign DNA with the same restriction enzyme.\n3. Mixture of foreign DNA with clipped plasmids.\n4. Addition of DNA ligase.\n5. Introduction of recombinant plasmid into bacterial cells.\n6. Production of multiple gene copies by gene cloning and selection process for transformed cells.\n7. Final screening for transformed cells.\n \n\n \nAdditional Methods for Analyzing and Cloning Nucleotide Sequences:\n1. Gel Electrophoresis. is used to separate either nucleic acids or proteins based upon molecular size, charge and other physical properties. Various DNA's can be identified by their characteristic banding patterns after being cut with their particular retraction enzymes. Genes can be isolated, purified and recovered from the gel with full biological activity.\n2. DNA Synthesis. It is possible to create known short DNA sequences in the laboratory. The Sanger Method is used to identify the sequence of a DNA molecule. This method is based on: a). use of restriction enzymes into small reproducible fragments. b). produce in a test tube DNA strands complimentary to a strand from the restr
1096803
Here are a few sites that have good ones :\nhttp://ghr.nlm.nih.gov/handbook/basics/dna\nhttp://www.elmhurst.edu/~chm/vchembook/582dnadoublehelix.html (more a ball and stick model)\nhttp://www.envirohealthtech.com/images/DNA.jpg (this one is just pretty)