how to cultivate tobacco plant using plant tissue culture technique?
547272
We did this in a lab I studied in in college.  You have to grow the cells in a stationary location with light.  Often they will differentiate on their own, but you may heve to use a root promoting auxin to get the plantlets to take root.  Once they do, you transfer them to a suitable growing mix and voila! you have a plant.\n\nIncidentally, we pioneered some work growing Niotiana tabaccum cells in suspension by continually agigtating them.  They will not differentiate if you do this.
246421
cloning\nstem cells\ngenetic manipulation
140134
some places store tissue which is capable of regrowing into the whole plant (e.g. meristem) in liquid nitrogen. They can get them regrowing after storage. \nBut every plant species (and even varieties) requires a different adapted protocol of how to best freeze them and thaw them. Usually freeze protectants (e.g. mannitol) are added before freezing them.
593312
I'm not sure what the question is? Are you looking for someone to write an entire grant proposal and post it here? I'm not sure if such a grant would be funded, because the means of preventing culture contamination are pretty well known: Work in a sterile environment (tissue culture hood). Spray everything with ethanol before you put it in the hood. Use disposable pipets, etc.
238502
yes you can use that method, however, you can't if you don't have a red rose already.  You must take a tissue (perferably the auxillary node) of a red rose plant and harvest that to grow the new red rose.
746255
Grafting is a method of plant propagation widely used in horticulture, where the tissues of one plant are encouraged to fuse with those of another. It is most commonly used for the propagation of trees and shrubs grown commercially. (Grafting is limited to dicots and gymnosperms. Monocots lack the vascular cambium required.)\n\nIn most cases, one plant is selected for its roots, and this is called the stock or rootstock. The other plant is selected for its stems, leaves, flowers, or fruits and is called the scion.\n\nIn stem grafting, a common grafting method, a shoot of a selected, desired plant cultivar is grafted onto the stock of another type. In another common form called budding, a dormant side bud is grafted on the stem of another stock plant, and when it has fused successfully, it is encouraged to grow by cutting out the stem above the new bud.\n\nFor successful grafting to take place, the vascular cambium tissues of the stock and scion plants must be placed in contact with each other. Both tissues must be kept alive till the graft has taken, usually a period of a few weeks. Successful grafting only requires that a vascular connection takes place between the two tissues. A physical weak point often still occurs at the graft, because the structural tissue of the two distinct plants, such as wood may not fuse.
76056
take your soil sample and suspend some in a little sterile water; plate out the suspension on agar plates in the usual way and isolate the colonies as a way to do a preliminary sort. Then maybe culture them ... Then do your in-depth analysis.
1150426
Plant tissue cultures isolated from even a single cell can show variation after repeated subculture. Distinct lines can be selected with their own particular morphology and physiology. It suggests that the tissue culture contains a population of genotypes whose proportion can be altered by imposing an appropriate selection pressure. This variation can be transmitted to plants regenerated from the tissue cultures, and is called somaclonal variation. It provides an additional source of novel variation for exploitation by plant breeders.
609782
root, stem, leaf and flower
173081
http://en.wikipedia.org/wiki/Plant_embryogenesis
658318
There are a few sites with PowerPoint presentations of tissue culture which include sugarcane but not exclusivly.\nI have listed them below:
24977
Yeah, that's good.  You could grow some type of plant in each and compare the results.  Some advice - you might want to specify your experiment a bit.  For example, a similar idea is to see whether potatoes grow without soil.  See the third site below.\n\nCheck out these sites.\n\nScience fair projects involving photosynthesis\nhttp://photoscience.la.asu.edu/photosyn/education/sciencefair.html\n\nAgricultural ideas for science fair projects\nhttp://www.ars.usda.gov/is/kids/fair/ideas.htm\n\nScience Fair Central\nhttp://school.discovery.com/sciencefaircentral/scifairstudio/ideas.html
916485
It'd be hard to isolate an individual cell, you can have a whole bunch of sells to study. For plants you can grow algae and for animal cells, you're an animal yourself swipe the inside of your mouth and you're bound to get a nice little sample of cells that you can study under the microscope. If you plan on doing any sort of prolonged experiment with em then you have to find a way to keep em alive. Algae are easy enough, not sure how you'd keep animal cells alive for long though.
277383
what are you looking for exactly. how to grow it at how . give me some details and iam sure i can help.
656205
Try the websites www.protocol-online.com & www.cellculturechat.com\nThe first has protocols for most scientific applications, the second is a forum specifically for cell culture scientists. Good luck!
885960
Technically and scientifically it is called as tissue culture which is the development of tissues from a single parental cell under aspartic labotary condition which leads development of a full organisms also.
1015056
http://images.google.co.in/images?q=rubber+cultivation&hl=en\nhttp://images.search.yahoo.com/search/images?p=rubber+cultivation&sp=1&fr2=sp-top&toggle=1&ei=UTF-8&fr=FP-tab-img-t-t400&ei=UTF-8&SpellState=n-783755765_q-cJFAOCqaDe0njIooYFZBIwABAA@@\n\nhope this helps\nmay i have 10 pts please...\n\nu can also go on the serch engines and in the images section type 'rubber cultivation'