what would i get after injecting rabbit globulin in a second rabbit?
440581
You will get a rabbit that is temporarily immune to the bacterial antigen that was injected into the first rabbit. This would be like giving a type O mother Rhogam.
358289
There;\n\nhttp://images.google.com/images?sourceid=navclient&ie=UTF-8&rls=GGLR,GGLR:2005-38,GGLR:en&q=intramuscular%20injecting&sa=N&tab=wi
892129
this question depends on the brown hamster. if its BB crossed with the white (must be bb) then you will get all brown offspring.  However if the brown parent is Bb, the the offspring will be 1:1.  Two white offspring and two brown offspring.
556113
If a foreign substance (an antigen) is injected into a vertebrate such as a mouse or a human, some of the immune system's B-cells will turn into plasma cells and start to produce antibodies that bind to that antigen. Each B-cell produces only one kind of antibody, but different B-cells will produce structurally different antibodies that bind to different parts ("epitopes") of the antigen. This natural mixture of antibodies is known as polyclonal antibodies.\n\nTo produce monoclonal antibodies, one removes B-cells from the spleen of an animal that has been challenged with the antigen. These B-cells are then fused with myeloma tumor cells that can grow indefinitely in culture (myeloma is a B-cell cancer). This fusion is done by making the cell membranes more permeable. The fused hybrid cells (called hybridomas), being cancer cells, will multiply rapidly and indefinitely and will produce large amounts of antibodies. The hybridomas are sufficiently diluted and grown, so that one obtains a number of different colonies, each producing only one type of antibody. The antibodies from the different colonies are then tested for their ability to bind to the antigen (for example with a test such as ELISA), and the most effective one is picked out.\n\nMonoclonal antibodies can be produced in cell culture or in animals. When the hybridoma cells are injected in mice (in the peritoneal cavity, the gut), they produce tumors containing an antibody-rich fluid called ascites fluid.\n\nIn the above process, one uses myeloma cell lines that have lost their ability to produce their own antibodies, so as to not dilute the target antibody. Furthermore, one employs only myeloma cells that have lost a specific enzyme (hypoxanthine-guanine phosphoribosyltransferase, HGPRT) and therefore cannot grow under certain conditions (namely in the presence of HAT medium). Fusions between healthy B-cells and myeloma cells are rare, but when one succeeds, then the healthy partner supplies the needed enzyme and the fused cell can survive in HAT medium. This is the trick to detect the successfully fused cells.\n\n\nPolyclonal antibodies are antibodies that are derived from different cell lines.\n\nThese antibodies are typically produced by immunization of a suitable mammal, such as a mouse or rabbit. An antigen is injected into the mammal. This induces the B-lymphocytes to produce IgG immunoglobulins specific for the antigen
492731
THANK YOU, MORE_EVIL!!!!!! Finally, someone uses some common sense with their answer to an immunization question. They told me in the hospital they do it because they don't know if I had it or not, which was insulting considering I've been married to the same man for 13 yrs. I refused it. But, yes, they do give it, at birth, 2 months and 6 months. \n   For your question, something caused my cousin to die at 4 months, it was written off as SIDS, but she died within 48 hours of receiving shots. I'm not sure which one it was. I know of three other infants who died with hours of their shots, too. It does happen, that's why I don't do them.\nHere's what I found on the possible side effects.\n\n"serious allergic reaction involving swelling of the mouth, breathing difficulties, low blood pressure, hives, weakness, an increased heart rate, dizziness and shock. If your child has any of these symptoms, call 911 or take her to an emergency room.
376418
If B type is Combined With B Nothing Will happen\nIf B type is Combined With A The Blood will not accept this combination and it may lead to Reaction in the Blood which may cause death\nIf B type is Combined With AB Nothing Will happen since AB is a Universal Acceptor\nIf B type is Combined With O The Blood will not accept this combination and it may lead to Reaction in the Blood which may cause death
585636
Do you have looked PubMed? "http://www.ncbi.nlm.nih.gov" There are 36 article for serum albumin and dansyly glycine. Maybe you can try to find it experimentally.
1054075
Look at http://ccnmtl.columbia.edu/projects/biology/lecture8/images/g3poxids.gif
356981
I've been reading up on this new research as well. I'm really excited about it and am curious to see how well it works in human trials. Unfortunately, it wouldn't be the first time something looks promising in lab animals but fails miserably in human trials.
375278
You can get protein poisoning. Seriously. For example if you out in the wild and the only animal you could catch is rabbit... because of the low fat content and they are so high in protein you can actually get poisoned over a couple months.
312357
Adverse Reactions: Mild local reactions consisting of erythema, pain and tenderness, swelling and induration at the injection site are common, usually self-limited and subside without treatment. \n\nUp to 70% of children receiving a booster dose at 4 to 6 years of age have been reported to develop local redness and/or swelling ³5 cm in diameter. This is generally self-limiting and subsides without treatment. \n\nPersistent nodules at the site of injection have occurred following the use of adsorbed vaccine, but this complication is unusual. Sterile abscess at the site of injection has been reported (6 to 10 per million doses). \n\nMild to moderate systemic reactions occur frequently following injections of this vaccine. These usually consist of one or more of the following symptoms and signs: temperature elevation ³38°C, drowsiness, fretfulness, anorexia, vomiting, irritability, persistent or unusual crying. These symptoms are most frequent during the first 24 hours following vaccine injection and may persist for 1 to 2 days.The incidence and severity of fever and irritability can be reduced by administration of acetaminophen (15 mg/kg/dose) at the time of inoculation and again 4 and 8 hours after vaccination. \n\nPersistent, inconsolable crying lasting 3 or more hours (1%) and high-pitched, unusual screaming (0.1%) have also been reported after DPT vaccination. Convulsions and a hypotonic-hyporesponsive state have each been reported to occur at a frequency of about 1:1 750 injections of DPT. Most convulsions are brief, generalized and self-limited, and are usually associated with fever. Neither febrile nor afebrile convulsions have been shown to be associated with subsequent seizure disorder. Complete recovery, with no persistent sequelae, has been observed on follow-up of children with hypotonic-hyporesponsive episodes or convulsions. (See also Contraindications and Precautions.) \n\nAlthough there has been a concern about the possible association of severe neurologic illness (including encephalopathy) occurring within 72 hours of the administration of pertussis-containing vaccines to previously healthy infants, the risk of an association is so small compared to the background rate for these types of events that the question of causation probably cannot be answered. \n\nReanalysis of the National Childhood Encephalopathy study (NECS) in the United Kingdom has failed to confirm that there was an increased risk of permanent brain damage following acute neurological illness occurring within 7 days of pertussis vaccination. Additional studies have also failed to demonstrate an association between pertussis vaccine and permanent neurologic sequelae. \n\nSudden infant death syndrome (SIDS) has been reported in temporal relationship to the administration of vaccines containing diphtheria and tetanus toxoids and pertussis vaccine (DPT). Review of the evidence does not indicate a causal relationship between DPT vaccine and SIDS. Studies showing a temporal relation between these events are consistent with the expected occurrence of SIDS over the age range in which DPT immunization usually occurs.
279154
The second one works really well, but not with all proteins.  I think the second one can look at several proteins at once too.  It's better suited for complex mixtures.
204557
No.  Your urine contains things that your body either couldn't use or didn't need so re-introducing those things a 2nd time provides no benefit whatsoever.  If your body had needed them, they wouldn't be being cast off in a waste product.
863864
Polyclonal\n\nPolyclonal antibodies are antibodies that are derived from several different t B-cell lines. The selections of antibody molecules bind to different parts of the antigen, with different binding affinities.  Within a polyclonal sample, some of the antibodies will be specific for the antigen with which the animal was immunised.  The rest of the antibodies have been obtained from meetings with other foreign antigens that the animal has been exposed to through its life time.\n\nThe production of polyclonal antibodies begins with the immunisation of an animal, usually a rabbit.  Once the rabbit has been immunised, it is restrained in a special cage and its ear is prepared for removing a blood sample from the animal.  A razor blade is used to cut the vein on the rabbit’s ear.  The blood is then collected in glass tubes so the blood can clot, as this will be more efficient and more serum can be obtained with minimal hemolysis.  When the blood clots a wooden applicator is used to rim the edges of the clot, this step is important, as it results in getting the maximum amount of serum from the blood.  The antibodies are then purified directly from the serum.\n\nThere are some advantages for using polyclonal antibodies, however there are also \ndisadvantages.  Some advantages of using polyclonal antibodies are:\n\n\nAdvantages\n\n* After as little as 2-3 months after the initial immunisation of the animal, multiple                   \n   subclass and high affinity polyclonal antibodies can be isolated.\n\n* Polyclonal antibodies are relatively inexpensive to produce.\n\n* From one immunised animal, large quantities of polyclonal antibodies can be          \n   produced. (up to 10mg/ml)\n\n* Polyclonal antibodies provide an overall look at an immune response, due to them  \n   containing an entire antigen specific population\n\n\nSome disadvantages from using polyclonal antibodies are:\n\n\nDisadvantages\n\n* To obtain the correct specificity, the antibodies have to be highly purified\n\n* The amount of antibodies that can be obtained are limited to the life span of the  \n   immunised animal \n\n* Polyclonal antisera recognises many antigenic determinants, making it difficult to \n   study individual parts of the complex antigens.\n\n* As a result of changes in antibody affinity, specificity, and subclass, different \n   bleedings have to be characterised individually\n\n\nMonoclonal\n\n\nUnlike polyclonal, producing monoclonal antibodies is a much more complex process.  Monoclonal antibodies are produce from one clone of cells rather than many different clones like polyclonal antibodies.  This results in the antibody molecules being exactly the same and they bind to the same antigenic site with identical binding affinities.  To produce monoclonal antibodies, clones of a single hybrid cell are formed in a laboratory.  This is achieved by fusing a B cell with a tumour cell.\n\nThe process involves repeatedly immunising an inbred mouse with an antigen.  After several immunisations the animal’s spleen is removed. The spleen is then removed as it is produces the B-cells. Due to the spleen cells not being able to be cultured, the cells are combined with myeloma cells. The combination results in the production hybridoma cells.  Hybridoma cells that produce antibodies that are not specific for the antigen are easily screened out with an ELISA, making it easier to find the antibodies specific to the antigen.  Once a selected, a single hybridoma line is injected into a healthy mouse.  The injection causes a tumour to grow inside the mouse.  This is due to hybridoma cells and myeloma cells being able to produce tumours.  As the tumour grows it produces a fluid which is full of monoclonal antibodies.  This fluid is known as ascites.  Monoclonal antibodies are produced due to the hybridoma cell being produced from a single B-cell.\n\nSome advantages of monoclonal antibodies are:\n\nAdvantages\n\n\n*   Antibodies with low–affinity b
260311
This depends on a lot- what type of insulin, why you are changing, what times he eats.  Talk to your vet.
215597
Hmm, you're right, the methods section of that paper is not very clear.  Anyway, check out the ref below.  Look midway through the 4th paragraph under Results, titled "Effect of recombinant Acrp30 infusion on hepatic glucose fluxes."  There could be other methods, but this is what I found.  Good luck.
1144527
I put on 2 stone! It was horrible,I ended up on the injection one!