Where can I find detailed and effective  DNA Isolation procedure from human leukocytes?
444842
check the journal of immunology
514947
Everthing you ever wanted to know about DNA.\n\nhttp://www.dnaftb.org/dnaftb/
446083
Try the Human Genome Project\nThere is some other project where you send them a splot of your blood and they tell you your entire family history and migration patterns and everything.\nIt's called genetic anthropology.\n\nSome scientists now believe that everyone can be traced back to 4 major groups of people or something like that.\n\nHere's the only link I found but there are better ones out there.
520769
Good news: You won't need to read a whole book to get even losts of details as well as the basics: google for a "polymerase chain reaction faq" on the web. Its less complicated than you think. \n\n\nWant to spend less time commuting ? Check out http://www.hallisystem.bravehost.com  \nWrite us.
1127492
These types of DNA are used to create a much more accurate diagnosis of DNA samples lef tfrom teh crime\n\nemail me a kkstrong0@yahoo.com if you have further questions and ill try to tell you what i know
355375
Go here. http://www.dnacenter.com/\n\nYou can't make them get it done, but you can trick them into giving you DNA by collecting a straw they have used or a cigarette butt or, obviously, a semen sample. Anything with hair, skin cells, or spit will also work. There is no age or time limit.
327006
You're trying to say you have a synthetic chromosome. I would check all the experiments for contamination, and check the cells for endogenous expression before jumping to that conclusion. There are a lot of hurdles to leap, as mentioned in the other posts.
16328
DNA testing can be pretty accurate, but it depends on how many sequences they test.  If you read my test procedure explanation you will understand why DNA is used.\n\nFirst, Carbohydrates and lipids are pretty much identical between all people.  A few people have metabolic disorders that result in a build up of certain carbs, but generally it would be useless to test for the specific carb or lipid present, because they are the same for everyone.\n\nProteins do differ among people, but not as much as DNA does.  DNA provides the information telling ribosomes how to put proteins together in people, but only a small portion of DNA is ever tranlsated to protein.  Most of your DNA is junk, and the big DNA differences between two people are in this junk DNA, so that is what is used for DNA testing.\n\nThis is an explanation of how it is typically done:\n\nThe way DNA testing works is by measuring the lengths of DNA segments between two endonuclease cleavage sites.  An endonuclease is an enzyme that will cleave DNA at a particular sequence of bases.  Some specific cleavage sites exist in the DNA of all people.  In between two specific cleavage sites, you may have a sequence of tandem repeat DNA.  For example le us say your DNA looks like this:\n(Cleave) gene1-TGTGTGTGTGTG-gene2 (Cleave)\nWe will denote this length of tandem repeat segment as (TG)6.  My DNA may have a tandem repeat segment may be (TG)10, in which case the segment of DNA between the two cleavage points is longer for me than it is for you.  This additional length also makes it heavier.\n\nNow let us make a suspend lots of copies of this particular segment of your DNA in a fluid and make another suspension with my DNA.  \n\nLet us also say that we know that everyone has a sequence of TG repeats at least 6 repeats long.  The complement to this sequence is:\nACACACACACAC -> (AC)6\nWe could create a radio labelled (AC)6 probe and sprinkle it into the suspension with your DNA, the suspension with my DNA, and a suspension with unknown DNA.  This probe would bind to the DNA in all three solutions, but my DNA segment would still be heavier than yours, because it contains 10 repeats, even if only 6 are bound to the probe.\n\nWe now place some of the suspension of your DNA at a point on an adsorbent surface.  The suspnsion will travel along the surface, but at some point the DNA will stick to the surface.  If it is heavier it will stick sooner.  With good equipment all DNA of a certain length will stick at the same spot (or short line)\n\nSince my DNA is heavier than yours, mine will travel less far.  The unknown DNA will travel either as far as your DNA does or as far as my DNA does, and that will tell whose DNA it is.\n\nThe way you view the DNA on the adsorbent surface is with a film that will darken when exposed to our radio labelled probe.  You place this film on top of the adsorbent bed with the DNA samples and three small spots (or short lines) will show.  The distance the unknown DNA travelled in comparison to your DNA and mine tells us whose DNA it is.\n\nInvestigators do this with evidence; only they use many different tandem repeat segments scattered throughout the genome.  The more segments used, the lower the probability of a coincidence.
104426
DNA is a good source of evidence for forensic experts, for doctors to identify cancer, recently used for SARS detection, to name a few .\n\nThere are techniques which are used to detect the presence of a specific sequence in a given DNA sample. PCR  or Polymerase Chain Reaction is one of them. here we amplyfy a specific sequence of DNA of interest. if the biological sample contains that DNA sequence then its amplified and can be visualized on a electrophoretic gel. DNA being negativey charged runs on an agorose gel under electric current towards the positive electrode and gets separated based on the size...slower the movement the bulkier the fragment. \n\nforensic experts use this to match DNA samples from site of crime to the suspect. this can also be used in case of paternity disputes, to detect the predisposition of a person to cancer etc etc.\n\nthere are many more techniques which you can search
62320
well, hope these help:\n\nhttp://www.bbc.co.uk/dna/h2g2/A388217\n\nhttp://www.chemistrysurvival.com/
393656
You have to send in a sample.  You wouldn't have to travel personally to a lab.  I'm assuming you mean some sort of DNA fingerprinting profile.  We're a long way from mail-in complete genomes.
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http://cellbio.utmb.edu/cellbio/nucleus.htm\nhttp://www.cdli.ca/~dpower/cell/nucleus.htm\nhttp://www.cellsalive.com/cells/nucleus.htm\n\nDo some research, we're not here to complete your homework for you.
308642
http://biotech.biology.arizona.edu/labs/DNA_extraction_onion_studt.html\n\nhttp://www.miramar.sdccd.cc.ca.us/faculty/dtrubovi/micro/microhandouts/oniondna.pdf#search='DNA%20extraction%20in%20onion%20cells' \n\nhttp://www3.iptv.org/exploremore/ge/Teacher_Resources/teaching_materials/lesson1_onion.cfm\n\nhttp://www.nal.usda.gov/bic/Education_res/protocols/onion.extract.html\n\nhttp://www.sidwell.edu/us/science/vlb5/Labs/DNA_Extraction_Lab/dna_extraction_lab.html\n\nhttp://www.accessexcellence.org/AE/AEC/AEF/1994/dollard_onionDNA.html\n\nand on and on and on.  Just do a google search on your project.
697436
are you asking about DNA extraction or plant (apple tree) cross-breeding?
281681
look up Restriction Enzymes
729453
K.C.'s answer works if you have a selective media that will work. Also (or in addition to that) you may want to plate out colonies and then PCR out a unique sequence or two.\n\nThis wouldn't be definitive but if you design your primers right it may work, but also may be very time consuming.\n\nAlso you may want to try restriction digests to remap your gene. This would work well, if you have a good map.\n\nGood luck, what a bummer
384036
Heres a cool one: http://gslc.genetics.utah.edu/units/activities/extraction/