can someone please explain how a cryo microtome works??
447611
http://en.wikipedia.org/wiki/Microtome\nhttp://en.wikipedia.org/wiki/Frozen_section_procedure\n\nusing a microtome is a method to cut e.g. biological tissue into very thin slices, much thinner than you could possibly do with a knife. In order to prepare your tissue for cutting it has to be hardened, either by fixation or freezing. If it is by freezing, you also would use a microtome which is sitting basically in a deep freezer to keep the tissue frozen while sectioning (cryomicrotome)
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I can give you the reference for the procedure that is easy and very effective. Use this article to get an idea:\nhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10829079&query_hl=1&itool=pubmed_docsum
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I used to freeze and donate birds to Carnegie Museums. Is this what you want? I do have a microscope, but not so good at dissection.
82213
what i read from a medical journal (i forget the name) is this: 1st isolate a donor cell by sucking cells through a tiny hole neddle, 2nd get unfertilzed egg from female, 3rd remove the egg nucleus,4th insert the the donor nucleus (male) to the female nucleus, 5th insert the egg to the womb
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This gives a detailed explanation of how they work.\n\nhttp://en.wikipedia.org/wiki/Bubble_light
338178
http://inventors.about.com/od/mstartinventions/a/microscope.htm
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oh this has been awhile....ok a block of wood, a couple nails, some tape, some wire, and a six? volt battery.\n\nyou are basicaly making one of the nails an electro magnet so that when the circuit is completed it comes on and pulls down one of the wires to the top of it making a clicking noise, open the circuit the wire will spring back up.  \n\nhere is a more complicated one that will send to another one\nhttp://www.apluschilddev.com/headstart/services/projects/easy_make_telegraph.htm
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Check out these lesson plans -- on the third page, they show a schematic which will give you an idea of what has happened:\n\nhttp://www.nbtc.cornell.edu/mainstreetscience/kitlib/getlessonplan.php?id=55\n\nhttp://www.psrc.usm.edu/org/Polymer%20Demos/Pdf/slime.pdf
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Please see the following dry ice website.\n\nPS - I am a licensed Professional Engineer.  I reviewed the following website and found the information quite adequate.  If you need additional information you just may need to do some work.
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"Current designs for moderately complex nanoparts, such as the Fine Motion Controller or the Neon Pump contain thousands of atoms.  Eventually nanoparts may move up to the scale of complexity and sophistication to tens to hundreds of thousands of atoms." \n"The prototypical "nanoassembler" is the "molecular manipulator" described in Nanosystems, Section 13.4:"\n\nIf this sounds interesting to you, you want to see this:\nhttp://www.nanoathome.org/General/
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The parts of the microscope\nAt the top of the microscope is the eyepiece or ocular - a cylinder containing two or more lenses to bring the image to focus for the eye. The eyepiece is inserted into the top end of the body tube. This design allows for different eyepieces to be inserted to give different magnifications. Typical values for eyepieces include X5, X10 and X20. At the lower end of the microscope tube are one or more objective lenses mounted on a circular nose piece. Each objective lens is cylindrical and is screwed into to the nose piece. Typical values of objectives are x5, x10,x20, x40, x80 and x100. Below the objectives is the stage which supports the specimen being viewed. In the centre of the stage is a circular hole through which light shines to illuminate the specimen. Below the stage, the light is provided and controlled in a variety of ways. At its simplest, daylight is directed via a mirror. Most microscopes, however, have their own controllable light source that is focused through an optical device called a condenser with diaphragms and filters available to manage the quality and intensity of the light.\n\nThe whole of the optical assembly is attached to a rigid arm which in turn is attached to a robust U shaped foot to provide the necessary rigidity. The arm is usually able to pivot on its joint with the foot to allow the viewing angle to be adjusted. Mounted on the arm controls for focusing are usually placed, typically a large knurled wheel to control coarse focusing together with a smaller knurled wheel to control fine focusing.\n\nCompound optical microscopes can produce a magnified image of a specimen up to 1000× and, at high magnifications, are used to study thin specimens as they have a very limited depth of field. Typically they are used to examine a smear, a squash preparation, or a thinly sectioned slice of some material. With a few exceptions, they utilize light passing through the sample from below and special techniques are usually necessary to increase the contrast in the image to useful levels (see contrast methods). Typically, on a standard compound optical microscope, there are three objective lenses: a scanning lens (5×), low power lens (10×) or sometimes medium power lens (20x), and high power lens (40×). Advanced microscopes often have a fourth objective lens, called an oil immersion lens. To use this lens, a drop of oil is placed on top of the cover slip, and the lens moved into place where it is immersed in the oil. An oil immersion lens usually has a power of 100×. The actual power or magnification is the product of the powers of the ocular (eyepiece), usually about 10×, and the objective lens being used.
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you have microtubules, microfilaments, intermediate filements and microtrabecular system. these are vastly different from each other. do you have any more specific question?
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Diaphragm (a.k.a. Iris):  Many microscopes have a rotating disk under the stage.  This diaphragm has different sized holes and is used to vary the intensity and size of the cone of light that is projected upward into the slide.  There is no set rule regarding which setting to use for a particular power.   Rather, the setting is a function of the transparency of the specimen, the degree of contrast you desire and the particular objective lens in use.\nRevolving Nosepiece or Turret:  This is the part that holds two or more objective lenses and can be rotated to easily change power.\nStage:  The flat platform where you place your slides.  Stage clips hold the slides in place.  If your microscope has a mechanical stage, you will be able to move the slide around by turning two knobs.  One moves it left and right, the other moves it up and down.
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Its tricky work, i need digrams to explain to you so your better just following this link, I think it will help you out.
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I assume you mean how does that work.\n\nMost liquids expand when heated and contract when cooled. The alcohol in thermometer will expand (rise) when heated and contract (fall) when cooled.
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For part 1, if the cell's diameter was 1/15 of the total field, and the field was 450 microns across, then the cell's actual diameter was 450 microns/15 = 30 microns = 3 * 10^-5 meters.\n\nThe diameter of this cell in the drawing is 0.5 cm = 5*10^-3 meters.  This drawing therefore has a magnification of (5*10^-3)/(3*10^-5) = 166.6666666...times.\n\nFor part 2, The magnification is given by (size in image)/(actual size), so we have that 45000 = (1.5 mm)/a, where "a" is the actual thickness.  Solving for a, we get a = 1.5mm/45000 = 3.333*10^-8 meters = 33.3 nanometers = 0.0333 microns.\n\n-----------------------\n\nAs for the question posed in the "additional details", when the magnification of a microscope is increased, the diameter of the field being imaged decreases (choice "A").
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The Beamsplitter (basically) splits the infrared light into two halves. One half of the split light beam travels to a moving mirror in the interferometer and the other half to the fixed mirror. Both beams are reflected back to the beamsplitter where they recombine. Half of this recombined energy (the interferogram) is transmitted to the detector. All of this occurs in on every stroke of the moving mirror. This data (collected in the time domain) is then transformed via the Fourier algorithms to the frequency domain. A series of these scans with nothing in the sample compartment constitutes the “background”, then the sample is added, scans collected again, and the sample collection is ratioed against the background collection and what is left are the infrared absorptions of the sample.