what are the different techniques available for analysing cytoskeleton proteins?
442497
Abstract. \n...membranes, microvilli, and membrane projections, and act as crosslinkers between the actin cytoskeleton and proteins of the cell surface...\nhttp://147.52.72.117/IJO/2002/volume21/number5/935-940.pdf \n\n \nforthcoming meetings\nAim: to learn techniques commonly used to analyse signal transduction ... and cell adhesion (cytoskeleton, cell adhesion proteins, extracellular\nhttp://www.usgeb.ch/skmb2005.html \n\n \nBTO\n...and function of proteins, methods of ... Cytoskeleton and cell motility. ... Describe and use structure determination techniques to analyse\nhttp://www.bto.ed.ac.uk/courses/year1/index0506.phtml \n\n \nRandall Division o f Cell and Molecular Biophysics\n...actin cytoskeleton during migration. I use a number of different advanced imaging techniques to analyse ... analysis of interacting proteins by\nhttp://www.kcl.ac.uk/depsta/biomedical/randall/mparsons.html \n\n \nGlossaire (ULg)\nAnkyrins. A family of membrane-associated proteins responsible for the attachment of the cytoskeleton. Note: membrane proteins \nhttp://www.ebm.lib.ulg.ac.be/prostate/gloss.htm \n\n \nThe role of the axolemma in the initiation of traumatically induced...\n...proteins. Transmembrane proteins such ... matrix to the cytoskeleton via the ... Biophysical investigations have used novel techniques to\nhttp://www.jnnp.com/cgi/content/full/64/3/285 \n\n \nThe tiniest parts of \n...are as dispart as the techniques and ... natural science, is to analyse the ... energy or information, signal transducers, cytoskeleton...\nhttp://www.uni-leipzig.de/~philos/psarros/preprints/the_tiniest_parts_of.html \n\n \nGenome Biology | Full text | Analysis and functional classification\n...as well as the development of new techniques for ... genes encoding cytoskeleton proteins (such as myosin, actin, UNC-87, troponin T) and\nhttp://genomebiology.com/2003/4/4/R26 \n\n \nStudy Abroad 2004 INTRODUCTION 2 Deakin University's unique Study \nUnit Handbook GUIDE TO UNIT DESCRIPTIONS ACD206/306 Dance Production and Analysis Second semester, 1 credit point Prerequisite ACD203 or Level One \nhttp://www.deakin.edu.au/international/Documents/Brochures/SAHandbook.pdf \n\n \nAdrian Harwood\n...genetic, biochemical and cell biological techniques to analyse the ... has both a cytoskeleton and a network ... SH2 domain proteins,\nhttp://www.ucl.ac.uk/LMCB/pages/harwood.html
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Mass-spectrometry (MS) is the only technique that gives true quantitative answers with extremely high certainty, but MS of DNA is uncommon and hard to do.  A more typical approach would be to "run a gel" separate the different fragments based on size: you have a porous matrix, you place the mixture of DNA pieces on one end, you apply a voltage.  Since the DNA is charged it migrates on the gel, but the bigger pieces are hindered by the matrix (the gel).  You then run a "standard" side-by-side with your unknown mixture--something with a mixture of a few fragments on known size.  Typically these "standard ladders" which are commercially available come in increments, e.g. 100 bp, 500 bp, 1000 bp, etc.  So you will be able to tell the size is between those fragment lengths (e.g. that a band from your unknown mixture is between 100 and 500 bp) with very high certainty, but you do not know the exact length with any certainty.\n\nAlternatively you can sequence DNA--this is very easy and cheap now-a-days, and has a very high-certainty.  So after doing a gel you can cut out the different bands and have them sequenced individually.  Both steps--the gel and the sequencing--are very routine, cheap, and reliable.
436829
What you are asking about is in the realm of surface science.  There are many analytical techniques and I will not try to go through all of them.  This is a good starting place: http://www.uksaf.org/tech/list.html
238844
you have microtubules, microfilaments, intermediate filements and microtrabecular system. these are vastly different from each other. do you have any more specific question?
634567
you use a 3d molecular bio particle scanner.  most of your high end medical research facilities will have one. just call and ask for scanning
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Magnetosomes are membranous bacterial organelles sharing many features of eukaryotic organelles. Using electron cryotomography, we found that magnetosomes are invaginations of the cell membrane flanked by a network of cytoskeletal filaments. The filaments appeared to be composed of MamK, a homolog of the bacterial actin-like protein MreB, which formed filaments in vivo. In a mamK deletion strain, the magnetosome-associated cytoskeleton was absent and individual magnetosomes were no longer organized into chains. Thus, it seems that prokaryotes can use cytoskeletal filaments to position organelles within the cell.
863141
Yes it is. There are drugs that target the microtubule structure as well as those that act on the actin based microfilament structure.
171613
Electrophoresis, x-ray diffraction (x-ray crystallography), chromatography (usually use for plant cell), centrifugation, microscope, and by using fluorescence technology
566223
There are several ways to do it.. \n\nYou can perform an ELISA (Enzyme linked immunosorbant assay) this technique involves the use of antibodies specific for the cytokine you wish to measure and then a secondary antibody to bind to the antibody against the cytokine which is tagged with an enzyme that can change colour when a certain chemical is added allowing the whole reaction to be quantified and measure the amount of cytokine being produced.\n\nAnother method to use is reverse transcriptase PCR - this measures the amount of mRNA made of the cytokine of interest.. However controversy exists over this method as it is not known if the amount of mRNA present is truly indicative of the amount of protein made.\n\nHope this helps..\n\nKatie
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Eukaryotic cell--use modeling clay.
379658
They are involved in signal transduction mostly.  They are involved at the cell membrane.  They phosphorylate Rho GTPases.
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Gel filtration is a type of size exclusion chromatography where your packing material is made of silica or a polymer containing a network of uniform pores.  Larger molecules cannot get into the pores so elute more quickly, smaller molecules the opposite.\n\nTo measure molecular weight of an unknown protein you would first need to produce a calibration plot by running through a series of standard proteins of known molecular weight and measuring the elution time for each.  You would then produce a calibration plot of log molecular weight versus elution time (note the log here).  Then finally you would run your unknown protein through, measure it's elution time and then use your graph to get its log molecular weight and hence its molecular weight!!
907507
nucleus, cytoplasm and protoplasm ....
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polyacrylamide Gel Electrophoresis
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Try this one: \n\nhttp://www.kendall-bioresearch.co.uk/class.htm
957912
Molecular Biology of the Cell
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Microtubules are protein structures found within cells, one of the components of the cytoskeleton. They have diameter of ~ 24 nm and length varying from several micrometers to possibly millimeters in axons of nerve cells. Microtubules serve as structural components within cells and are involved in many cellular processes including mitosis, cytokinesis, and vesicular transport.