lactobacillus count media and method, How do I get a help?
437173
You will need a specific growth media for lactobacilli called Rogosa. If you think you want to count other lactic acid bacteria, e.g. enterococci, pedioccoci, streptococci, then use MRS (deMan Rogosa and Sharpe) media instead.\nCollect your sample (0.1 or 1 g), mix with a saline solution in a 1:9 ratio (10x dilution); then make serial dilutions taking 1 mL aliquotes and tranfering to new tubes with the 9-mL saline solution. This way your dilution tubes will have a 10-fold decreasing concentration of bacteria. Finally you pipet 1 mL from each tube and plate with the appropriate media. Incubate at 34C for 48hs and count the plates with colonies between 30 and 300, to reduce your error. If you count 100 colonies from an aliquote that was diluted 3 times, you'll have 100x10x10x10=100,000 colonies per mL from that tube. Make sure you consider previous dilutions when preparing the sample.
21641
yogurt with active live cultures,read the label
816577
I generally follow the recommendations provided by our safety officer at my academic lab.\n\nIf your liquid cultures can afford the extra volume, you can just add a lot of deionized h2o, that should stop the growing (by lysing the cells).  Then toss in the bio-hazard bag, for the autoclave.  \n\nOr, if you have access to an autoclave, then autoclave the culture, and you can probably dump down the drain.\n\nCheck with your safety officer!!\n\nIt was fun thinking about your question.  Pass on the good Karma!
292071
What class was this in? What grade level? What text were you using?  I may be able to help you if I know the context in which the question was raised. I have a feeling that I am not getting all the information you had. Please tell me and maybe I can help. I am an old chemist and microbiologist.
928716
1.What bacteria are we talking about (E.coli? Which strain?)\n2.There is no such standard solution for any type of bacteria.\n3.Why on earth would you need such a dilution? \n4.What type of facilites do you have access to (You can isolate single cells by FACs provided you have a proper marker and this expensive equipment)
194727
I use azo yeast. you find it at your local drug store near the monosat and things. It is an herbal remedy u take 3 times daily. I use that and eat 3 servings of yogurt with live cultures *check the label to make sure* also limit your dairy and sugar intake during this time
701203
macroscopic. you "farm out" the bacteria on a agar plate, then describe what grows (usually after 48 hours, but can both be more or less- depending on the species)\nNote: there are VERY many possible culture media (usually Agar based), and some bacteria will grow only on a few of them- that helps with the ID\nAlso- some bacteria need special culture conditions- eg. anaerobes- which die if they are cultured in contact with air. Also helps with the ID.\n\nMicroscopic. First of all look at the bacteria under a microscope- that gives you some info.- eg are they round (cocci) or elongated (bacili) or curved (vibrio) or spiral, or any of a multitude of shapes.\nsome bacteria are transparent and can only be seen in a "dark field" (reflected light from bacteria seen on an otherwise unlighted space) typical for Treponema pallidum- causes syphilis.\n\nThen the bacteria can be stained- very many possible stains and methods- but the most commonly known is the Gram stain (hence some bacteria are called "Gram positive" or Gram negative" - depending if the stain "caught" on the cell wall.\nSome stains are then removed with acid preparations- and in some bacteria it works, in others not. eg. the TB bacteria are known as "acid fast"- meaning an acid bath does not remove the colour of the original stain\n\nEssentially you are supposed to go through all the steps and look up in a book what fits your particular bug.\nThe bug can also be a fungus- yeast or mould
54264
take some LACTOBACILLUS powder supplement. drink lots of water. consult a Doctor if u get bloody diarrhoea.\n\nIt will help restoring the good bacterial flora inside your gastrointestinal tract.
57968
If you are trained in microbiology you can tell by eye.  Plate out the bacteria (for single colonies) onto a universal media that is suitable for your target bacteria,  ie CLED, Maconkeys, Blood, Chocolate.  Incubate until there is a growth (usually 24hrs at 37 degreees celcsius is aerobic).  Look at the plate, are the colonies uniform in appearence.\n\nIf you are using liquid culture youu could use microscopy before plating out.  Either a gram stain or a direct (wet prep). This would let you know if you had all gram positives or all gram negatives or all rods or all  cocci or a mixture.  You would still have to plate out to make sure only one colony type grows.
130049
Find a petri dish and some bacteria culture.\n\nSwab your mouth.\n\nRub it across the culture.\n\nLet it sit in the incubator for 24 hours.\n\nAnd voila!\n\nAll the microogranisms in your mouth.
580834
Here is a link: \n\nhttp://www.scienceprojects.com/dairybacteria.htm\n\nwww.bacteriamuseum.org is a great resorce for Micro.
912037
Find a question that relates to the local area you live in, something you may have noticed, or has controversy.  A saying I like is "One measurement is worth a thousand expert opinions", which is the essence of science.  So find something to measure.  When I was in school (a long time ago) people I knew wondered about whether the local creek was clean enough.  Kids played in the creek and some people fished in it, but I thought it shouldn't foam and have a not quite clear look.  So, I got some sterile petri dishes from the biology lab, got some q-tips, and talked the local hospital pathologist into helping me.  If you need help, ask, you might be surprised, it turned out he was curious too.  Anyway, I learned a bit about how to identify bacteria and yes, the creek was polluted-bad!  I made the front page of the local paper.  I'm not saying do this, but think of a local problem and measure it.  Hope this helps.
340737
I have the same problem and i have found that soluble fiber works the best.  The only place that I have been able to find it is online at helpforibs.com, you can order it from there.  Also there are some recipes and other things that might be helpful if you haven't already seen the website.
330994
Take frequent, small sips of water or a rehydration drink and small bites of salty crackers. \nTry to increase your fluid intake to at least 1 qt (1 L) per hour for 1 to 2 hours or until diarrhea symptoms disappear. \nBegin eating mild foods the next day or sooner, depending on how you feel. \nTry yogurt that contains live cultures of Lactobacillus. Not all forms of yogurt do; read the label carefully. Frozen yogurt does not contain Lactobacillus. \nAvoid spicy foods, fruits, alcohol, and caffeine until 48 hours after all symptoms have disappeared. \nAvoid chewing gum that contains sorbitol. \nAvoid dairy products for 3 days after symptoms disappear. \nNonprescription medication may be helpful in treating your diarrhea. Use nonprescription antidiarrheal medication if you have diarrhea for longer than 6 hours. Do not use nonprescription antidiarrheal medications if you have bloody diarrhea, a high fever, or other signs of serious illness.
839821
Checked my bacteria program on my PDA and all that came up where....neisseria gonorrhoeae, neisseria meningitidis, moraxella catarrhalis and veillonella sp. \n\nFirst off, all I recall about those organisms is that they are aerobic cocci that are gram-negative. The exception being viellonella sp. as it is an anerobe. \n\nSecondly, I only looked for those traits I just mentioned, no idea if any of these meet your other criteria. \n\nThirdly, haven't you been doing tests all semester to narrow it down? The pathogens are limited to what you have studied, so it is not as the scope is that large. Try making a list of all of the organisms you have studied, turn it into a graph with all of the traits possible from all of the various tests...start filling in the blanks and you should have no problem figuring out which organism it is. Our specimen was contaminated and I found no problem determining both the assignment and the contaiminant using this method. \n\nPerhaps it is one of the organisms I mentioned above, it's very likely if you have studied any of them in Microbiology. But if not, use the method I described above and it really is just a process of elimination. \n\nGood luck
852489
Print off the picture, cut out all areas that bacterial growth is observed and using a sensitive balance scale weigh the paper that has bacterial growth on it.  Works pretty good.
337160
Congrats, I am working on my thesis too.  There is PDA (potato dextrose agar), V8 agar (made from guess what, V8) and many others.  Each is designed for a specific purpose.  I am not an expert on this, but try a search engine.