What is fibroblast protocol culture from human skin?
442554
Primary cultures or immortalized cells?\nI use CF3 and they grow just fine in DMEM + 10% BCS, they have contact inhibition, so you need to split them twice a week (if they become 100% confluent, they will detach from the dish).
413250
Skin, lungs, and liver.
799301
hope you will get something useful in this link\nhttp://en.wikipedia.org/wiki/Human_cloning
1115957
Not with current freezing techniques. Too much cell damage. In the future I think that a process will be possible. Will it be ethical or moral in a rapidly overpopulating world will be the ultimate question.
899552
Hello, and what an interesting question.  \n\nMy answer is not about cloning humans, but rather cloning cells from humans that have the potential to turn into specialized cells.\n\nCorporate support of cloning human cells (like all Pharmaceutical companies supporting drug development) would only result in treating "end stage" support for diseases. \n\nThis is not a good thing.  Here is the reason: \n\nCorporate support generally does not advocate a fundamental understanding of how the disease begins, and it would not support understanding how to prevent the disease. \n\nThe best reason to support human cloning, in my opinion, is to understand disease and to prevent it. This has nothing to do with profits. \n\nIf Corporate companies supported preventative medicine, then they would make small profits in the long run.  \n\nBut when they support "end stage" therapies, their profit potential is limitless.\n\nNowadays Corporate profits can be hidden in money driven governmental policies (I live in the USA), by making human cell cloning sound wonderful and very humanitarian (and costly).  \n\nBut the real point is missed by consumers (who pay taxes which would offset tax benefits given to corporations anyways). \n\nThe real point in human cloning should be in preventing the disease in the first place.  And that is the real potential in human cloning of cells.  \n\nMaybe there are some humanitarians and good doers out there that really see this potential, and when you find them, they probably have nothing to do with corporate industry (or politicians or universities).  \n\nMy suspicion is that the humanitarians are being silenced throughout this money making frenzy for human cell cloning.  \n\nAs this happens, a shift towards "end stage" treatment will be emphasized, and understanding of the biology behind the disease will be suppressed (or, worse, even patented).  \n\nThis philosophy of treating diseases (as opposed to preventing them or even curing them) does have its advantages.  \n\nFirst, you won't have to change your lifestyle.  \n\nSecond, if you work in the field (as I do) then you can be assured of a long career supported by government and corporate entities and monies.  \n\nIt was fun thinking of our future.  I hope I helped you out!
169320
I think he was talking about technologies where they can grow human parts on rats and such using stem cells...like grow ears and human skin on host animals that can be harvested for transplants and such.  Not sure how this is too different from doing transplants with pig hearts and such....
239043
assuming they are ingrown hairs....exfoliate.
72038
Ask a certified dermatologist.
153377
http://www.webmd.com/hw/skin_and_beauty/hw65255.asp
599793
To isolate it, take a sample, dilute it then take a sample from that & spread it on a sterile petri-dish.\n\nYou can then take a sample from a pure culture.
620737
http://diwww.epfl.ch/lami/biovision/ \n\nread this hear
350803
library.thinkquest.org/ 19347/media/humancell.gif
780969
Question: Where are you going to find an authentic sample of his cells?\n\nNo, it's not ethical, but it would most certainly be done if the scientists thought they had an authentic viable sample. \n\nPeople would kill to get their hands on it. One to protect the sample from being debased by science, another in the belief it will heal or provide immortality, one to see if the clone would have perfect morality and miraculous powers, one to prevent the sample or clone from becoming an object of worship, one to keep Jesus a mystery only attestable to by faith, one to prevent the other from getting it... each would have thier own motivation.
273694
The trick is deceptively simple.  A microbe is chosen--often a very fussy strain of Saccharomyces or Escherichia coli--and the DNA that encodes human fibrinogen is introduced into its genome.  This engenders the production of fibrinogen by the microbe; that fibrinogen can then be harvested and purified.\n\nThe same technology is used to produce human insulin, human growth hormone, and a wide array of other polypeptides useful to human medicine.\n\nWhat I find fascinating about it all is that the vat fermentation production system used to produce all this is a spinoff of the vat fermentation system used in the first mass production of penicillin, developed at the USDA's Agricultural Research Lab in Peoria, IL (Now known as the NCAUR) around the time of WWII...
951092
a group of cells form a tissue
131337
Nov. 4, 2004 -- Balding men and women take note. Hair cloning -- the next hair restoration remedy -- is on the way.\n\nOK, it's not exactly cloning, although that's what it's come to be called. Researchers working to perfect the new technique prefer the term "hair multiplication."\n\nAnd no, it's not ready for prime time. Not yet, says Ken Washenik, MD, PhD. Washenik is medical director for Bosley, the giant hair restoration company that's one of several firms racing to bring hair multiplication to market. He's also clinical assistant professor of dermatology at New York University Medical Center.\n\n"There is no doubt it will be a tremendous breakthrough," Washenik tells WebMD. "It is the thing people have been waiting for. There have been so many remedies for hair loss that didn't pan out. This is one that really looks like it is going to happen -- and happen in the next few years."\n\nIt's not just hype, says hair researcher George Cotsarelis, professor of dermatology and director of the hair and scalp clinic, at the University of Pennsylvania School of Medicine in Philadelphia. Cotsarelis consults for Bosley, but is not involved in the company's research program.\n\n"It is hard to predict whether they will be successful, but there is good evidence that will happen," Cotsarelis tells WebMD. "It is not quackery -- they are not charlatans. It is based on real scientific knowledge. But there are a lot of hurdles still to overcome."\n\nThe promise of early research often evaporates in the harsh light of clinical testing. Yet Washenik predicts that hair multiplication will be available for hair restoration in three or four years.
1061989
Fibrosis stands for scar tissue.