what are the results when gram stain is performed on saccharomyces cerevisia and penicillium chrysogenum?
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Aren't most molds Gram negative (Pink)?
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It all depends on what kind of stain it is.  Go to "Google search" with that information, and you'll find a load of websites withanswers for you.
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Preenrichment- for non fat dry milk and dry whole milk, weigh 100g of product and transfer to 1L distilled water containing 2ml of 1%aqueous brilliant green dye.Incubate at 35C for 24 +/- 2hrs. Enrichment- transfer 1ml of pre enrichd smple to 10 ml selenite cystine broth and tetrathionate broth respectively incubate at 35C for 24 +/- 2hrs.streak from enrichment media to the ff diff media: brilliant green, salmonella-shigella and bismusth sulfite incubate at 35C fo 24 +/- 2hrs.\nappearance of typical colonies:\nBGA- colorless, pink to fuchsia, translucent to opaque with surrounding medium pink to red.\nSSA- uncolored to pale pink, opaque, transparent or translucent; some produce black centered colonies.\nBSA- brown black sometimes with metallic sheen.\nsubject for further identification using  TSI agar for confirmation.streak the slant and stabb the butt. salmonella positive TSI cultures have alkaline red slants and acid (yellow) butt with or without H2S (blackening of agar) =)
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It's the cell wall. The peptidoglycan in the gram positive bacterial cell wall retains the crystal violet and make them look purple in color.
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Gram-positive bacteria :\nGram-positive bacteria are classified as bacteria that retain a crystal violet dye during the Gram stain process. Gram-positive bacteria will appear blue or violet under a microscope, whereas Gram-negative bacteria will appear red or pink. The difference in classification is largely based on a difference in the bacteria's cell wall structure.\nThe following characteristics are generally present in a Gram-positive bacteria:\nA very thick cell wall (peptidoglycan) \nIf a flagellum is present, it contains two rings for support (an additional two rings are not necessary, unlike in Gram-negative bacteria, because the thick cell wall is supportive enough) \nTeichoic acids are present, which serve to act as chelating agents, and also for certain types of adherence \nIn the original bacterial phyla, the Gram-positive forms made up the phylum Firmicutes, a name now used for the largest group. It includes many well-known genera such as Bacillus, Listeria, Staphylococcus, Streptococcus, Enterococcus, and Clostridium. It has also been expanded to include the Mollicutes, bacteria like Mycoplasma that lack cell walls and so cannot be stained by Gram, but are derived from such forms.\n\nThe actinobacteria are another major group of Gram-positive bacteria; they and the Firmicutes are referred to as the high and low G+C groups based on the guanosine and cytosine content of their DNA. If the second membrane is a derived condition, the two may have been basal among the bacteria; otherwise they are probably a relatively recent monophyletic group. They have been considered as possible ancestors for the archaeans and eukaryotes, both because they are unusual in lacking the second membrane and because of various biochemical similarities such as the presence of sterols.\n\nThe Deinococcus-Thermus bacteria also have Gram-positive stains, although they are structurally similar to Gram-negative bacteria.\n\nBoth Gram-positive and Gram-negative bacteria may have a membrane called an S-layer. In Gram-negative bacteria, the S-layer is directly attached to the outer membrane. In Gram-positive bacteria, the S-layer is attached to the peptidoglycan layer. Unique to Gram-positive bacteria is the presence of teichoic acids in the cell wall. Some particular teichoic acids, lipoteichoic acids, have a lipid component and can assist in anchoring peptidoglycan, as the lipid component is embedded in the membrane.\n\nGram Negative Bacteria:\nGram-negative bacteria are those that do not retain crystal violet dye in the Gram staining protocol. Gram-positive bacteria will retain the dark blue dye after an alcohol wash, whereas Gram-negative bacteria do not. In a Gram stain test, a counterstain is added after the crystal violet, which colors all Gram-negative bacteria a red or pink color. The test itself is useful in classifying two distinctly different types of bacteria based on structural differences in their cell walls.\n\nMany species of Gram-negative bacteria are pathogenic, meaning they can cause disease in a host organism. This pathogenic capability is usually associated with certain components of Gram-negative cell walls, in particular the lipopolysaccharide (also known as LPS or endotoxin) layer.\nThe following characteristics are displayed by Gram-negative bacteria:\n\nCell walls only contain a few layers of peptidoglycan (which is present in much higher levels in Gram-positive bacteria) \nCells are surrounded by an outer membrane of lipopolysaccharide outside the peptidoglycan layer \nPorins exist in the outer membrane, which act like pores for particular molecules \nThere is a space between the layers of peptidoglycan and the secondary cell membrane called the periplasmic space \nThe S-layer is directly attached to the outer membrane, rather than the peptidoglycan \nIf present, flagella have four supporting rings instead of two \nNo teichoic acids are present \nLipoproteins are attached to the polysaccharide backbone whereas in Gr
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it's called a gram stain.  if the microbe stains, then it is said to be gram positive... if it doesn't stain (or stains poorly), then it's gram negative.\n\nyou have to use all the stains to complete the gram staining process
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Some unstained cell structures are hard to tell from other similar cell parts unless you ues a differential stain.\nA differential stain can be made in 2 steps.\nFirst step blue, stains one part\nSecond step red, stains the other.\nThis can also be helpful if a suspect bacteria does or does not take up a stain. This can help to identify a bacteria.\nMedical clinics do this all the time in cbc's (complete blood count), and throat smear analysis.
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Yeast can cause some pain.
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Oil immersion objective has got magnification of 100X and used for observing slide which are Gram Stained.  Gram staining is nothing but a technique of staining bacteria to confirm their Gram Nature i.e. Gram positive or Gram Negative\n\nhttp://biology.clc.uc.edu/fankhauser/Labs/Microscope/Oil_Immersion.htm\n\nhttp://www.microscopy-uk.org.uk/mag/indexmag.html?http://www.microscopy-uk.org.uk/mag/artmar02/pjoil.html
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Yeasts make up a group of single-celled (unicellular) fungi.  They react with chemicals.\nAn example with glucose as the substrate is\nC6H12O6 (glucose)    to    2C2H5OH + 2CO2
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you can surely rule out some things based on the type of plate you are using.  assuming that you have covered that base, you can separately pick a colony that you think may be a contaminant and look for differences in growth patterns, color, morphology, gram stain, growth on other media, and if all else fails, you can sequence it to find out exactly what it is.
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Yeast has the ability to enzymatically convert simple sugars into Ethyl alcohol and Carbon dioxide.\n\nNormally this is a two step process.  The first step is the aerobic budding and multiplication phase where the yeast grows in numbers.  The second is an anaerobic phase where the actual fermentation takes place.\n\nThis is true for both baker's yeast (to leaven breads) and brewer's yeast (for alcohol production).  Yeasts are single-celled (unicellular) fungi, not bacteria.
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Gram staining is a laboratory test used for identifying various kinds of bacteria.  Some kinds of bacteria react to the stain and those are gram positive, the rest don't and they are gram negative.  Different kinds of antibiotics are designed to kill bacteria that are gram positive, gram negative or both.
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The Ziehl-Neelsen stain, also known as the acid-fast stain, was first described by two German doctors; Franz Ziehl (1859 to 1926), a bacteriologist and Friedrich Neelsen (1854 to 1894), a pathologist. It is a special bacteriological stain used to identify acid-fast mycobacteria. It can also be used to stain few other bacteria like Nocardia. The reagents used are Ziehl-Neelsen carbolfuchsin, acid alcohol and methylene blue.
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I believe it is the smaller grains that make the difference - the grains are much tinier than regular yeast.   This site should help.
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We do.  We run tests on other carbohydrates such as lactose, maltose, and sucrose, as well as different kinds of amino acids and fats.\n\nFor instance - Different species of Neisseria ferment glucose, (like that bacteria that causes gonnorhea)  with other members of the same family don't ferment glucose (like Moraxella cattarhalis).  Microbiologists can identify some bacteria by their fermentation of different carbs.
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Gram Positive bacteria have a different membrane structure than gram negative bacteria.  This results in a different uptake of a staining techinque where gram positive bacteria stain pink while gram negative stain purple.