what is the use of RT PCR?
79133
It is used to quantify how much of a mRNA transcript is in a sample. RT is for reverse transcriptase, which is used to make a cDNA (complimentary DNA) copy from the mRNA (messenger RNA) template. DNA is stable, whereas RNA is not. The cDNA can then be subjected to PCR using the primers of your choice to amplify your transcript of interest.\n\nThe point is that this tells you how actively a gene is being transcribed in a given cell under given conditions.
115714
This product has everything you want and more including AM, This is a great product.\n\nhttp://www.icomamerica.com/products/receivers/pcr1000/
469125
A link to a protocol from Alpco Diagnostics:\n\nhttp://www.alpco.com/pdfs/30/30-2201.pdf
960362
Im not sure what u mean by RTD. But maybe this might help..\n\nhttp://www.answers.com/RTD
865567
The Only Way to Know For Certainty, that There is Active HCV Replication, is to Do a RT-PCR, for the Viral RNA.  Not a Simple Test for the Antibody (Serology).
191717
This is the melting temperature for a particular oligonucleotide. This temperature is important for producing a successful polymerase chain reaction product. Depending on the sequence of your oligomer primer you will have to use various temeratures to ensure that they anneal properly to the target sequence.
558244
Im pretty certain that word is just a slightly different version of the rtf specification so you should be able to parse most of it with rtf.\n\nHope that helps.
827602
You're doing the PCR reaction to look for an amplification of a DNA segment.  When you run it out on a gel and look for the presence of bands of DNA, water will be your negative control because there is no DNA.  You want to do this to have something to compare your amplified sequence with; you also want to do this to make sure that bands in your amplified lanes are due to PCR and not to something DNA that was present in the water.
66018
RTF, or Rich Text Format, is a very compatible format and is even human readable. Because it can be used by so many programs (including use on the Macintosh platform), it cannot use traditional compression technicques employed by similar file types (such as .DOC).
671418
PCR is a technique in which a specific stretch of DNA is amplfied greatly (a billion-fold or more).  Briefly, two small stretches of cDNA are designes that will bind to a stretch of DNA-almost like bookends. Then a Taq polymerase is added, which produces a complete cDNA starting from each primer.  Then the solution is heated, and the strands melt apart, then it's cooled, new primers bind (now there are twice as many stretches of that DNA) and the reaction is run again- Each time the reaction is performed, it doubles the amount of that specific DNA strand.  "Taq" polymerase is special in that it comes from a bacteria that lives in extremely high temperatures, so the melting phase of the reaction doesn't denature it.
838778
To be honest with you i don't quite understand your question, it is often that restriction enzymes are used to create genomic library for which you look into the overlapping sequence and then be able to determine which fragment is next to which. If that is what you have done, you should have used the fragmented DNA sequence to determine the orginall DNA sequence that you digested it with the enzyme, to confirm the sequence you ahve found is correct, you can use PCR, you got the hypothetical sequence, thus by making primers from the start and the end of the sequence (doesn't matter which way round) you should have amplify the same sequence which you started with, you got the amplified DNA (same as the orginal DNA because you used primers that complemented the very start and the very end of the sequence which you found out after the restruction enzyme mapping) run it on gel electrophoresis with a DNA size marker that will include the size of DNA you have sequenced, if the amplified DNA size is the same as the sequence you have found from restriction mapping, that means your primers did complemented from the start and the end, that means the sequence you thought is actually the correct sequnece.\nI am sorry, i don't think i am making this clear enough, but anyway, that's the best i can think of.
322699
RTF stands for rich text file.\nYou can open it using wordpad\n\nClick Start -> Programs -> Accessories -> WordPad\n\nIn WordPad click File -> Open ;then open the file.\n\nAlternatively Microsoft Word can also open this file.
281294
yes, the quality might not be great but polymerase chain reaction can increase the amount of DNA to an amount that can be used for analysis.
1177839
it used to be isolate the pathogen from an infected host and use it to infect a healthy animal, but i believe they are now using the third choice more because the second choice doesn't always work.  so i would say isolate bacterial/viral proteins by electrophoresis.
414422
PCR involves\na) marking a region of DNA with borders (also called primers), say A and B, and then\nb) amplifying the DNA flanked by these primers. \n\nFor DNA finger printing, the A and B are chosen such that the, region between them varies in length across individuals. This is because certain elements (i.e., stretches of nucleotides) present between the A and B vary in their number. So, when PCR is performed, the product length varies depending on the template DNA taken from different individuals. \n\nBy the electrophoresis technique, the PCR products of different length can be visualized and one can detect the differences between the individuals. Thus, the VNTR (variable number of tandem repeats) among individuals is the basis of DNA finger printing by PCR.
684354
http://www.tsha.utexas.edu/handbook/online/articles/RR/rnr5.html
137653
There are different ways to sequence cDNA, probably the most common right now is PCR based:  an unlabeled primer is put into a PCR reaction with the CDNA, fluoroescent molecules attached to dNTPs (a different color for each) are added along with non-labeled dNTPs.  Addition of the fluorescent dNTPs stops the reaction so that different length fragments with different dNTPs at the end are obtained.   By analyzing the different lengths of these fragments by electrophoresis and looking at the color of the dNTP terminator, the sequence can be obtained.