what dangers should I be aware of when employing different detachment techniques for cell passaging?
86077
You should get the MSDS sheets for the chemicals that you use, try invitrogen.com this is the best advice without more details on the techniques you're using.\nThere are two techniques I've used, one is detachment using trypsin/EDTA and the other is scraping the cells off the side of the flask. In both methods there is little danger from the tools you use, more danger is posed by the cells themself. If you're using an established cell line, get the details on it from www.atcc.org if not, be extra careful!
745931
Hey Christopher!\n\nI think the most common adverse event reported with the external catheters is UTI's... Best way to avoid infections and UTI's is to keep the area and the unit CLEAN!  :)\nOnce you switch, get the manufacturer name, and model of your cath, and the website should provide you with infomation that was reported by people that used the cath while it was still in testing.\nAlso, are you a member of a group or website for paraplegics?  You should join a chat room, where you can talk to others.  You might find a broader audience of paraplegics in that type of forum, who can provide you with their actual experience.\n\nBest of luck to you  :)
911724
Risks?\nAs with any surgical procedure, there is some risk involved. Serious complications are rare but could include anesthesia reactions, infection, transfusion reactions, or injuries at the needle insertion sites. The NMDP and its centers take all the necessary precautions to ensure the safety and well-being of the donor. A number of personal health questions are asked when individuals join the Registry to be sure they are in general good health. Before actually becoming a donor, a volunteer will receive a thorough physical examination.\n\nDoes marrow donation hurt?\nFollowing the procedure, donors can expect to feel some soreness in the lower back for a few days or longer. Some donors have also reported feeling fatigued and having some difficulty walking.
178960
NOt a great idea,a there is a balloon attached inside, so pulling it out without deflating could be harmful.  \n\nAre finished with the tube, want a new one, can you eat without it?\n\nActually, the 800 nurse or nurse on call will probably walk you through it.  Removing the tube is not especially hard to do, you do not want to invite infection though, that is a concern.  It needs to be as clean as clean can be, you could faint, so it is best to have someone there.  It is a strange sensation. I am assuming it is a PEG tube.\n\nHere is one guy's story of peg removal:\n\nMember # 4 \n\n \n--------------------------------------------------------------------------------\nRemoving the peg takes about 30 seconds; I actually removed my own since I was in Santa Fe, a long ways from MDACC. Some like mine have a little thin wire in them that causes the other end to "pigtail" up once they have put it in. Cut that little wire and the tube inside straightens out and you just pull it out. A 2X2 gauze and a piece of tape for one day covered things up, and that was it. No leaks, no fuss, no muss. Other types have a small, inflated balloon at the end that keeps them in. Deflate the balloon and the tube comes right out. While I am not advocating that you take your own out, I mention all this to illustrate that it is no big deal. I had mine in for about 8 months after the completion of all the treatments... it was that long before I was able to get enough nutrition through my trashed mouth and throat. I'm glad to hear that your mouth hasn't developed mucositis yet, but in my case it really didn't show up until about the third week. Also, many get a longer course of radiation than 4 weeks, and with your short schedule, you may not have as many of the side effects that you read about from others here. Let’s hope that is the case.  \n\nHere is more: \nTube Feeding \n\nWhen surgery or treatment for oral cancer affects the patient's ability to eat, a feeding tube is inserted to facilitate meeting nutritional needs. First introduced in 1980, today more than 200,000 patients every year receive this form of therapy. The location of oral cancers, and the resulting damage to the oral / esophageal tissues from treatments, makes weight maintenance and obtaining proper nutrition especially difficult.  \n \n\nFeeding tubes may be inserted through the nasal passageway for short-term use, but for those patients who require longer use of the tube, it is customary to place the tube directly into the stomach through the abdominal wall. This second method is called a percutaneous endoscopic gastrostomy (PEG) tube. The feeding tube may also be put in place in anticipation of other treatments such as radiation or chemotherapy, allowing the patient to adapt to its use prior to treatments. Feeding tubes are not painful and are not easily visible when wearing normal clothes. When not in use, they can simple be taped to the patients stomach to prevent them from moving around under clothing. \n\nPEG tubes are placed with the aid of an endoscope, the scope going down the throat to assist in guiding the placement of the tube through the wall of the stomach. The surgery is simple and involves little risk or discomfort. The procedure takes about 20 minutes. The PEG tube extends from the interior of the stomach to outside the body through a small incision only slightly larger than the tube itself in the abdominal wall. The tube is prevented from coming out of the stomach by one of several methods. Some brands have a small wire within the tube, which after insertion is pulled from the exterior end of the tubing causing the portion within the stomach to curl up or “pigtail,” preventing it from being pulled out. Other systems employ a very small balloon at the end of the tube which is inflated within the stomach after insertion, serving the same purpose. Removal of the tube simple involves cutting the wire which created the pigtail, or deflating
107841
there won't be any problems.\njust need to be a bit careful for some time after the operation.
214979
You can check out this site, it explains a step by step technique:\n\nhttp://www.pall.com/biosepra/34696_7290.asp
143429
I don't have statistics for you, but this is a known complication.\n\nSpeaking very, very roughly, I'd guess that in the facility where I work, it occurs to fewer than one person out of a hundred.  I realize that's no consolation when it happens to you.\n\nIn my experience, it usually doesn't require surgical repair.  The monitoring you describe sounds more typical.\n\nFor what little it's worth, I'm sorry this happened to you.
738710
I have a couple of concerns about this rubber band technique:\n1. The question has not been asked as to why you have a gap. Some conditions, such as gum problems, will be made worse by this technique.\n2. There is a risk that the rubber will slide under the gums and go down the roots. This will lead to irreversible damage to the bone supporting the teeth. Be VERY careful that this doesn't happen. I have seen this happen with a standard rubber band, and there's no real difference here.\n3. The results may not be permanent because the teeth may move back after you remove the rubber. And if you keep doing this over and over again in this uncontrolled manner, you may cause long-term damage.\nAs they always say, "If it looks too good to be true, it probably is."
392297
There are many procedures with which to trypsinize cells. All include washing the cell monolayer with TD, or in rare cases, with VE. This removes serum, which contains trypsin inhibitors, and lowers the concentration of both calcium and magnesium, both of which inhibit trypsinization.\n\nI like to rinse the cells with TD, add approximately 1 ml of straight trypsin, rock the dish, aspirate a majority of the trypsin, and then incubate the dish at 37 °C until the cells come off the dish.\n\nSome people put 1 ml of 5 fold diluted, with TD, trypsin on the dish and leave it on. Others do this using full-strength trypsin.\n\nCells vary enormously in how fast they come off the dish. Some cells come off within one minute, some require 15 min. Cells should be trypsinized until they come off the dish, but not longer. Don't rush, but don't over do it.\n\nCells that are extremely hard to dislodge with trypsin should be rinsed with VE prior to trypsinization and then trypsinized in the presence of VE. The EDTA promotes disaggregation. Unfortunately, EDTA is toxic to cells at high concentration--it deprives them of Zn++.\n\nIt is essential to inactivate trypsin and to remove EDTA after you have gotten the cells off the dish. Trypsin inhibition is accomplished in part by trypsin inhibitors in the serum in the growth medium. Bovine serum is a rich source of trypsin inhibitors.\n\nThe best, but most laborious way to reseed cells, is to dilute the suspended cells with medium containing serum and spin them down in the gray table top centrifuge--5/8 speed, 4 min. Then aspirate the supe, resuspend the cells in medium, and reseed them.\n\nA short cut that works with cells that are being seeded at a low cell density is simply to dilute the trypsinized suspension of cells with medium and seed the cells directly.\n\nIf the cells are to be reseeded at a high cell density, the trypsin will not be diluted or inactivated sufficiently and the cells will not attach.\n\nThis is not satisfactory when VE is used because EDTA is growth inhibitory even at low concentrations.\n\nIt is also not satisfactory with chick cells, since they are grown in medium containing only 2% serum and cannot be seeded very sparsely.
356289
Not sure about the vacuum- I'd be more concerned about the possibility of needing a tissue... at either end. *sigh* The scientific mind never stops wondering...even when it should. lol.
584044
it doesn't sound safe. I don't even know what it is. But if you have to ask, the answer is no.\n\nhuffing kills your brain.
280375
Compound W works well just keep it off the surrounding tissue.
1151179
"Human cadaver grafts and animal tissue grafts, although approved for use by the FDA, carry warnings: They are not guaranteed 100-percent risk-free from transmitting diseases to patients. Furthermore, these grafts have never been proven more effective than autografts.\n\n"The safest and best surgical procedures are those transplanting the patient’s own tissue (autograft). Just as people bank their own blood before a non-emergency medical surgical procedure, there is no risk of disease transmission when patients use their own tissue.\n\n"When patients have insufficient protective gum tissue, we recommend gingival augmentation or root coverage procedures using autografts. We advise against using human cadaver or animal tissue grafts."
661934
http://www.ask.com/web?q=why+is+it+important+to+avoid+contact+of+KMnO4+and+ethanol%3F+&qsrc=0&o=0
203956
Sterile technique and Universal precautions. You should not being doing this. A trained healthcare worker should. What are you doing this for?
471559
Ask a specialist or a person whose job is to do such things.\nSorry, but I do not believe anyone here can be precise.
528811
dudue take this seriuosly "you'er detoriating!"\nok just kidding,you shed dead cells evryday (yes cell are to small to see), here's the catch sometimes our skin just peels as an new layer takes it's place, so hurray you got new skin! ok that was too hype but it's normal, it could be that or an alergic reaction to detergent, dishwashing liquid or any thing, but if it doesn't hurt, itch or bleed then there's nothing to worry about