What is the procedure for doing the the Sheep Rosette test.?
88540
E-rosette test\nn.\nA test to identify T cells by mixing purified human blood lymphocytes with serum and sheep red blood cells; rosettes of red blood cells form around human T cells on incubation.
323353
Duck test.\n\nIf it looks like a duck, waddles like a duck and quacks like a duck-it's a duck.
878741
There are certain steps to perform a hypothesis test.\n\n1. Construct a null hypothesis Ho .\n2. Construct a alternate hypothesis H1.\n3. Decide test statistics and parameters [this includes degrees of freedom, significance value] [inmost cases its denoted by Z or t].\n4. Compute the p-value.\n5. Check with the available table.\n6. make inferences wether the hypothesis is accepted or not.\n\nThere is no fixed fomulae for this, but the choice of null hypothesis and test statistics depends on the problem. \n\nThe type of null hypothesis depends on the probelm(wether certain parameters are given or certain distributions are followed by given data etc) and the test statistics depends on parameters.\n\nI hope now you know it.\n\nAll the best
497593
Test it for esteroids
466802
High-throughput DNA Isolation Protocol \n\nCTAB Method \n\n1.      Add 80 ul 2-mercaptoethanol to 40 ml CTAB buffer (see recipe below) right before use (0.2%) and mix. \n\n2.      Preheat CTAB DNA isolation buffer to 65°C in a water bath. \n\n3.      Grind freeze-dried leaf tissue (~100mg) in a Mixer Mill for 3-5 min at 30 time/sec with one 3mm steel bead in a 1.1 ml  tube strip from Qiagen\n\n4.      Carefully pour beads out of tubes and add 300 ul CTAB buffer into each tube with a multi-channel pipette (if a plate is used, you can leave beads in until the end of work)\n\n5.      Cap the tube strips and label each strip, caps and rack, incubate the samples in 65 °C water bath for 60-80 min, use shipping tape to secure the rack and lid to prevent caps from openning up while inverting the rack to mix samples.\n\n6.      Mix samples every 15 min by inverting rack 5-10 times to make sure plant tissue mixes well with the buffer. Watch for sample leaking from caps. \n\n7.      Take samples out of water bath, briefly spin sample box in a centrifuge for 1 min at 1500 rpm (balance box weight before spinning), separate caps from tube and put the cap strips on clean paper and arrange them in order so that they can be reused. \n\n8.      Add 300 Chl/IAA (25:1) into each tube with a multi-channel pipette, cap tube with original cap strips, put a plastic board between tube cap and rack lid to make sure every strip cap gets the same pressure from the lid, cover the box with lid, secure the lid with shipping tape. Mix the samples by inverting the box slowly for 15 min (Chloroform can create pressure and cause leaks of chloroform, so the tubes have to be tightly capped).\n\n9.      Centrifuge at 5,700 rpm  for 10 min. \n\n10.  Transfer the aqueous phase using a wide-bore pipette tip (about 200ul) into a labeled clean plate (650 ul plate), add 150 ul of cold isopropanol (-20 °C), securely cover the plate with a plate cover (or cap strips) and mix gently by inverting the plate several times to precipitate DNA. \n\n11.  Recover the DNA by centrifuging at 5,700 rpm (6100g) for 10 min. Discard the supernatant immediately by suddenly inverting the plate and dry it briefly on several layers of paper towels by briefly touching the plate on paper towels two to three times on different positions of towel to remove excess liquid. This step should be very quick (a few seconds). Then turn the plate back upright and air-dry samples to remove excess liquid for ~10 min.\n\n12.  Add 500 ul of 70% Ethanol directly to the pellet, cover the plate, and invert it gently several times to wash  for 5 min. \n\n13.  Centrifuge at 5,700 rpm for 8 min, and quickly discard wash buffer as described previously (check DNA pellet in each well to make sure DNA stayed in the bottoms of wells\n\n14.  Air-dry DNA pellet at room temperature for ~20 mins to remove excess liquid.\n\n15.  Re-suspend the DNA pellet in 200 u1 of ddH2O and leave it at 4°C overnight \n\n16.  DNA concentration will be ~100ng /ul and can be determined using either an agarose gel or a spectrophotometer. \n\n \n\n                                                                            CTAB  DNA Isolation Buffer (500 ml) \n\n \n\n2% (w/v) CTAB (sigma)\n 10.0 g\n \n1.4M NaCI\n 40.91 g\n \n20 mM EDTA\n 20 m1 of 0.5 M EDTA \n \n100 mM Tris. HCI (pH 8.0)\n 50 ml of 1.0 M\n \nAdd ddH2O to make a final volume of 500 ml \n \n\n \n\nSDS Method \n\n1.      1-2 young leaves (~100mg) are placed in a labeled 1.1-ml collection tube or in a plate well, freeze-dried, and ground for 3-5 min at 30x/sec to a fine powder using a Mixer Mill and one 3-4mm steel bead in each well. (To isolate DNA from dry seeds, half to 3 seeds are wrapped with a weighing paper, crushed with pliers, placed in a col1ection tube or   in a deep-well plate ).\n\n2.      Preheat extraction buffer to 65°C in a water bath. (If the tissue samples are stored at -20°C, allow them to warm up to room temperature).\n\n3.      Add 0.2% (v/v) ß-mercap
508478
Test the Hypothesis\nThis is the part where the experimenter actually completes hands-on tests to either prove or disprove the hypothesis. To truly test the hypothesis, the experimenter must compare the results of the experimental group compared to a control group where the variables have not been changed. Example: For two weeks, a group of people eat a certain amount of chocolate each day and another group eats no chocolate.
858344
I am assuming that your sheep pen must be rectangular, guessing from your use of length and width, and you are working with a set perimeter value.  In this case, your pen should be a square of length and width each equal to 1/4 of your total perimeter.  However, if you are free to maintain a pen of any shape, you get the most area per unit perimeter in a circle, of radius = 1/(2pi) times the perimeter.
160136
Chromotropic acid method. Although the atricle deals with textile testing, it may as well work with foods.\n\nhttp://www.ijdvl.com/article.asp?issn=0378-6323;year=2004;volume=70;issue=6;spage=342;epage=344;aulast=Rao
363177
Hello there,\n\nhere's a website that describes the entire procedure:  http://www.gicare.com/pated/epdlv02.htm
807570
yourquestionwouldappeartobeincompletepleaserestateandmaybewecanhelpyouormaybeyoucouldtry\n\nhttp://www.nal.usda.gov/fnic/foodcomp/search/
376995
This will guide you through the entire process. Good luck!\nThe Church Library: An Outline of Procedure\nMain Page & Introduction\nhttp://www.acu.edu/academics/library/cfm/churchlibrary.html
909092
G'day nursyuhada m,\n\nThank you for your question.\n\nA sheep pen is used to hold sheep when the farmer needs to keep them together. This includes times such as shearing or drenching or other times when neccessary.\n\nI have attached sources for your reference.\n\nRegards
809519
Well, let's see.\n\n18 = b + s + c,  where b means bird(chicken)\n\ntotal number of legs:\nLt = 2b+4s+4c, right?\n\nLt = 3Ht +10 , total legs 10 more than 3 times number of heads\n\nI'd assume each animal only has one head, thus Ht = 18\n\nLt = 3*18+10 = 64\n\nLike most problems of this sort you must find integer solutions.\n\n18 = b + s + c\n74 = 2b + 4s + 4s\n\nA trial and error procedure is effective.  \nstart at \nb=s=c=6, -> Lt = 12+24+24 = 60, need more legs!\nb,s,c\n5,7,6, -> Lt = 10+28+24 = 62,\n4,7,7 -> Lt = 8+28+28 = 64 <------------- bingo\n\nNote that you can freely shift sheep and cows as long as the total is 14.  So, there are other solutions:\n\n4,14,0 -> Lt=8+56 =64....no cows.....
934689
its a test.. will you go for it
999035
Here is a page showing which test is appropriate for what:\n\nhttp://www.socr.ucla.edu/Applets.dir/ChoiceOfTest.html
273925
The simplest way is spectography. if you are speaking of a breathalizer test it is via conductivity.
88021
Using a set of callipers if the droplet is large enough. If not then using an eyepeice micrometer and a microscope.