what are the basic requirements of tissue culture?
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A standard tissue-culture laboratory should provide facilities for: (1) washing and storage of labwares; (2) preparation, sterilization and storage of nutrient media; (3) aseptic manipulation of plant material; (4) maintenance of cultures under controlled conditions, (5) observation of cultures; and (6) acclimatization ...
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3D Cell Culture Techniques The reason for the growth in 3D cell culture is it's simply a better way of representing human tissue outside the body. 2D cell cultures only exist in two dimensions. That's not an accurate representation of how cells grow or how they are affected by disease and injury.
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For growth and multiplication of bacteria, the mimimal nutritional requirements are water, a source of carbon, a source of nitrogen and some inorganic salts. Phototrophs are bacteria that derive energy from sunlight while chemotrophs derive theirs from chemical reactions.
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Cells divide when: an organism grows. organisms need to replace worn-out cells. organisms need to repair damaged tissue.
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Living tissue is made up of cells. There are many different types of cells, but all have the same basic structure. Tissues are layers of similar cells that perform a specific function. The different kinds of tissues group together to form organs.
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['Thick keratinlike coat.', 'Peptidoglycan.', 'Cell membrane.', 'A small amount of cytoplasm.', 'Very little water.', 'Bacterial DNA.']
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The tissue culture laboratory must have four basic environments: an office, a washing and media preparation room, a transference room, and a culture room, which require a minor to major grade of cleaning, as well as a minor to major restriction on personnel access.
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The principal environmental requirements for plant growth include adequate space for root and canopy development, sufficient light, water, oxygen, carbon dioxide, and mineral elements, and temperature suitable for essential physiologic processes.
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Totipotency include tissue culture which is a process by which a new plant part can be formed by a small tissue of that plant. It helps to make the more amount of plant thus help in propagation and improvement.
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Cell culture is a technique where cells are grown under laboratory conditions in vitro. Tissue culture is a technique of growing tissue by transferring them into an artificial environment in which they can continue and function.
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Cell Culture Products. Cell culture is defined as the removal of cells from their natural environment and placed into a satisfactory in vitro environment in a laboratory for further study. ... Effective growth factors to maintain phenotype and overall cell health.
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['One. Cells are the basic structure and function of a living thing.', 'Two. All organisms are made out of cells.', 'Three. Only existing cells can make new cells.']
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['Roots. Roots are simple tissues that are derived from the stem of the plant. ... ', 'Xylem. The xylem is tissue that transports water throughout the plant. ... ', "Phloem. The phloem is the plant's food transportation system. ... ", 'Leaves. ... ', 'Growth.']
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['Connective tissue ranges from avascular to highly vascular.', 'Composition: Composed mainly of nonliving extracellular matrix that separates the cells of the tissue.', 'Location: It is present in between different tissue and organs. It can be found in and around the body organs.']
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['whether they are made of prokaryotic or eukaryotic cells.', 'whether the cells are living singly or organised into multicellular and thus complex organisms.']
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Tissue culture can create a plant directly, whereas micropropagation must use tissue cultures to create a new plant. ... However, tissue culture is used to produce plants with a small amount of tissue from an already existing plant's growing tips.
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['movement (which may occur internally, or even at the cellular level)', 'growth and development.', 'response to stimuli.', 'reproduction.', 'use of energy.', 'cellular structure.']