how to prepare dpph solution?
446516
Hence to make a 0.1 mM solution , you will need to dissolve 39.4 mg of DPPH in methanol/ethanol. A simple way to check is for 1 M in 1000 mL you need 394 g, for 1 mM you need 394 mg, so for 0.1 mM you would require 39.4 mg. However, I would suggest making a higher stock 10 mM and then dilute it before use.
1189536
['120ml of PVA glue (½ cup)', '½ Tbsp of bicarbonate of soda.', '½ cup of shaving cream (not gel)', '1 Tbsp of contact lens solution (remember, one with boric acid, like Renu MPS)', 'Food colouring (optional if you want to make coloured slime)']
1298423
The highest activity was achieved in the 7.0–8.0 pH range for ADH, and around 7.0 for AldDH. The optimum temperature for ADH lay between 35 and 40 °C and at about 35 °C for AldDH.
771124
['Solution.', 'Step 1: Separate the half-reactions.', 'Step 2: Balance elements other than O and H.', 'Step 3: Add H2O to balance oxygen.', 'Step 4: Balance hydrogen with protons.', 'Step 5: Balance the charge with e-.', 'Step 6: Scale the reactions so that they have an equal amount of electrons.']
1235259
The ABTS•+ and DPPH assays are widely used methods for the assessment of the antioxidant capacities of natural products, they both are spectrophotometric techniques based on quenching of stable colored radicals (ABTS•+ or DPPH) and show the radical scavenging ability of antioxidants even when present in complex ...
684437
Prepare 1 mL of SDS lysis working buffer (100 mM Tris pH 6.8, 2% SDS, 300 mM NaCl, 50 mM DTT). The volume required will depend on the total number of cells for 10,000 cells per µL and may be more than 1 mL. a. Dissolve one vial of No Weigh DTT in 50 µl of lysis buffer stock.
1223688
The assay is based on the measurement of the scavenging capacity of antioxidants towards it. The odd electron of nitrogen atom in DPPH is reduced by receiving a hydrogen atom from antioxidants to the corresponding hydrazine (Contreras-Guzman and Srong 1982).
749823
Dissolve potassium iodide in about 200 cm3 distilled water and then add iodine crystals. Make the solution up to 1 litre with distilled water. It is essential to prepare it 24 hours before it is required, as iodine is slow to dissolve.
1087399
['Always fill the tank before adding RxP, then add one full bottle, ... ', 'Drive out each tank down to less than 1/4 (one quarter)-tank.', 'Do that twice, then skip adding RxP at the third fill-up.', 'Add RxP in the fourth fill-up, and then every other fill-up after that.']
180970
['Tertiary butylhydroquinone. ... ', 'Magnesium carbonate. ... ', 'Erythosine (Red #3). ... ', 'Propylene glycol. ... ', 'Ferric orthophosphate.']
393640
dithiothreitol (DTT or Cleland's reagent) may be used at a final concentration of 350 mM (54 mg/ml). Dilute 1 part sample with 1 part Laemmli sample buffer. More sample buffer can be added if necessary.
1109202
NPH +Regular insulin: Always draw the Regular (clear) insulin into the syringe first. Phosphate-buffered insulins ( NPH insulin) should NOT be mixed with lente insulins. Zinc phosphate may precipitate, and the longer-acting insulin will convert to a short-acting insulin to an unpredictable extent.
532540
c) Preparation of Thidiazuron The stock solution was prepared by dissolving 10 mg of thidiazuron (TDZ) in 1 ml of DMSO and the volume was made upto 100 ml with sterile distilled water and used in 6 different concentrations (0.5, 1, 1.5, 2, 2.5 and 3mg/l).
352698
['HOT WATER - 250ML. [00:24]', 'BAKING SODA - 1 TBS. [00:28]', 'MIX UNTIL DISOLVED AND COOL OFF. [00:32]', 'CLEAR GLUE. [00:44]', 'COLD WATER - SAME AMOUNT AS GLUE. [00:52]', 'CONTACT LENS SOLUTION. [01:04]', 'SLOWLY POUR GLUE INTO BAKING SODA WATER. [01:16]', 'MIX SLOWL. [01:29]']
1455586
['N-Acetyl-Carnosine (NAC) 1.0% (approved by IVP)', 'Glycerin (lubricant) 1.0%', 'Carboxymethylcellulose sodium (lubricant) 0.3%']
1133789
['Lbs/day = MGD x (ppm or mg/L) x 8.34 lbs/gal. % purity.', 'GPD = MGD x (ppm or mg/L) x 8.34 lbs/gal. (% purity) x lbs/gal.', 'GPD = MGD x (ppm or mg/L) x 8.34 lbs/gal.', 'ppm or mg/l = lbs/day. or.']
645325
['Wet Film Thickness.', '=', 'Dry Film Thickness x 100.', '% Volume Solids.', 'Spreading Rate (m2/litre) =', '% Volume Solids x 10.', 'DFT Required.']