what is the difference between a simple and differential stain quizlet?
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Simple stain uses 1 dye to increase contrast of cells. A simple stain determines size, shape, and arrangement of cells but cannot differentiate between types of bacteria. A differential stain uses 2 or more dyes to differentiate between organisms or between cell structures.
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Giemsa stain is also a differential stain, such as when it is combined with Wright stain to form Wright-Giemsa stain. It can be used to study the adherence of pathogenic bacteria to human cells. It differentially stains human and bacterial cells purple and pink respectively.
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Difference Between Simple And Complex Tissue. The key difference between simple and complex tissue is, simple tissue is made up of only one type or kind of cell whereas complex tissue is made up of different type of cells. ... Example blood tissue. Below are some other differences between the two tissues.
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Acid fast stains are used to differentiate acid fast organisms such mycobacteria. Acid fast bacteria have a high content of mycolic acids in their cell walls. Acid fast bacteria will be red, while nonacid fast bacteria will stain blue/green with the counterstain with the Kinyoun stain.
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Stain or dye is the synthetic chemical which is derived from nitrobenzene or aniline. stains are used commonly in microbiology to increase the contrast between microorganisms or parts of its and the background,so that it can be easily visible.
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Individual bacterial cells are nearly colorless, making them difficult to see under the light microscope. ... Methylene blue is a simple stain that colors cells blue. In a negative staining technique, a negatively charged stain colors the background, leaving the cells light colored and unstained.
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There is a difference. Stains are made of colored pigments that stick in the grain and pores of the wood surface while dyes consist of microscopic particles that penetrate the wood itself. ... When mixed with the proper solvent, dye crystals dissociate into individual molecules.
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A differential stain is a specific type of staining that allows for microbe identification, and distinguishing between cells in a mixed sample. ... Simple staining involves adding a basic, cationic dye to the organism. The positive dye is attracted to the negative cell wall and cytoplasm, resulting in stained cells.
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Quartz is stain-resistant, while Dekton is stainproof. Quartz is vulnerable to heat; Dekton is not. Quartz often becomes discolored when exposed to the sun, whereas Dekton is UV-proof. Quartz shouldn't chip, while Dekton is known to be vulnerable to chips and even splitting.
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What is the difference between direct and indirect staining? Direct staining is done with basic dye while indirect staining is done with acidic dye. Using an indirect stain allows you to see the bacterial cells much more accurately, as well as a clearer shape and size.
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Best Answer: The difference is a simple microscope has one lens where a compound microscope has an objective lens and an eyepiece with a longer focal length. a simple microscope only has one type of lens, usually objective lens, but a compound microscope had both objective lens and ocular lens.
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India ink is a negative stain that colors the background brown, leaving the cells bright and visible. A differential staining technique is a procedure that allows the observer to visually distinguish between different types of bacterial cells based on the idea that not all cell types stain equally.
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The key difference between simple and compound epithelium is that simple epithelium has a single layer of cells while compound epithelium has more than one cell layer. There are two main types of epithelial tissue based on the number of cell layers. ...
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Translucent stains are most similar to the aesthetic provided by interior wiping stains. ... Semi-transparent stains offer a much wider color pallet than translucent stains. They cover the wood a bit more than translucent stains, but the wood can still be seen.
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The gram-positive bacteria retain the crystal violet colour and stains purple whereas the gram-negative bacteria lose crystal violet and stain red. Thus, the two types of bacteria are distinguished by gram staining. Gram-negative bacteria are more resistant against antibodies because their cell wall is impenetrable.
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A simple stain will generally make all of the organisms in a sample appear to be the same color, even if the sample contains more than one type of organism. In contrast, differential staining distinguishes organisms based on their interactions with multiple stains.
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The difference lies in the amount of pigment in each. Transparent stains, although they appear clear, have some pigment in them for UV protection. Semi-transparent stains have more pigment, but still allow some of the wood grain to show through. Solid stains are basically like paint.