what is the difference between direct and indirect methods of measuring cell growth?
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The direct method involves serial dilution and standard plate count measurements where the weight of microbial cells is correlated with cell noses. ... Indirectly, microbial growth is measured by measuring the dry cell mass or turbidity of the sample in a spectrophotometer.
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As a cell grows bigger, its internal volume enlarges and the cell membrane expands. Unfortunately, the volume increases more rapidly than does the surface area, and so the relative amount of surface area available to pass materials to a unit volume of the cell steadily decreases.
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BMR may be measured by gas analysis through either direct or indirect calorimetry, though a rough estimation can be acquired through an equation using age, sex, height, and weight.
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Direct transfer can occur when someone has touched an item. Indirect transfer is when DNA from someone ends up on an item without them having touched it. Such indirect transfer has been demonstrated. ... Furthermore, it has also been shown that DNA can be transferred during the forensic examination of items.
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In absolute quantification using the standard curve method, you quantitate unknowns based on a known quantity. ... In relative quantification, you analyze changes in gene expression in a given sample relative to another reference sample (such as an untreated control sample).
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The main difference between direct and indirect heat transfer systems is the expenditure of air required to achieve heat transfer. Chilling, heating, processing and cleaning air results in high energy consumptions in direct heating processes.
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Growth in Stems The increase in length of the shoot and the root is referred to as primary growth. It is the result of cell division in the shoot apical meristem. Secondary growth is characterized by an increase in thickness or girth of the plant. It is caused by cell division in the lateral meristem.
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The main difference between growth and development in biology is that growth is the increase in size and mass of a particular organism over a period of time whereas development is the overall changes in the whole organism in terms of organization and function.
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What is the difference between growth and direct growth? Growth means investing in growth option through a regular plan, while direct growth means investing in growth option scheme through a direct plan.
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What are the disadvantages? Advantages: direct method of cell population determination, counts both viable and nonviableDisadvantage: hard to count with motile cell 3. Dyes such as methylene blue is used for differentiation between viable and non- viable cells. 4.
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Cell proliferation is the process where cell increase in number as a result of cell growth( replication of genetic materials) and cell division( governed by cell cycle). ... Cell differentiation is the process of changing one cell to another cells type. Each cell is regulated by specific gene expression.
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One significant difference between growth phases is that the first growth phase is about cell growth while G2 is about cell division.
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The levels of expressed genes may be measured by absolute or relative quantitative real-time RT-PCR. Absolute quantification relates the PCR signal to input copy number using a calibration curve, while relative quantification measures the relative change in mRNA expression levels.
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Growth means investing in growth option through a regular plan, while direct growth means investing in growth option scheme through a direct plan. What is the difference between growth and dividend plan?
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Direct analysis refers to those procedures that detect the specific disease-causing mutations or foreign DNA sequence. ... Indirect detection methods, on the other hand, are utilized when the sequence of a disease-associated gene or disease-causing mutation is not known.
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While direct and regular plans are two different channels of investing, growth and dividend are two profit distribution options of a scheme. ... “Direct growth” means investing in growth option of a scheme through a direct plan, while “Growth” means investing in growth option of a scheme through a regular plan.
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Total and direct bilirubin levels can be measured from the blood, but indirect bilirubin is calculated from the total and direct bilirubin. Indirect bilirubin is fat-soluble and direct bilirubin is water-soluble.