what are the applications of southern blotting?
14967
Southern blotting is used in a number of applications. The primary usage of Southern blotting is to identify a specific DNA in a DNA sample. It is mostly used in the identification of viral infection and certain bacterial infections.
825113
['Alpha-fetoprotein (AFP) test or multiple marker test.', 'Amniocentesis.', 'Chorionic villus sampling.', 'Cell-free fetal DNA testing.', 'Percutaneous umbilical blood sampling (withdrawing a small sample of the fetal blood from the umbilical cord)', 'Ultrasound scan.']
1265175
The technique may use PCR, size determined by gel electrophoresis, and Southern blotting to produce a pattern of bands unique to each individual. Therefore, VNTRs are being used to study genetic diversity (DNA fingerprinting) and breeding patterns in animals.
503989
VNTRs are an important source of RFLP genetic markers used in linkage analysis (mapping) of genomes. They have become essential in forensic crime investigations. The technique may use PCR, size determined by gel electrophoresis, and Southern blotting to produce a pattern of bands unique to each individual.
683788
Antibodies to Bcl-2 can be used with immunohistochemistry to identify cells containing the antigen. In healthy tissue, these antibodies react with B-cells in the mantle zone, as well as some T-cells. However, positive cells increase considerably in follicular lymphoma, as well as many other forms of cancer.
1371891
Tests that may be used to diagnose URIs are: Throat swab: Rapid antigen detection can be used to diagnose group A beta-hemolytic strep quickly. Lateral neck X-rays: This test may be ordered to rule out epiglottitis if you have difficulty breathing. Chest X-ray: Your doctor may order this test if they suspect pneumonia.
382304
Tandem dimer Tomato (tdTomato) provides a useful alternative to enhanced green fluorescent protein (eGFP) for performing simultaneous detection of fluorescent protein in histological sections together with fluorescence immunohistochemistry (IHC).
1254451
Description: BMS147 specifically recognizes Annexin V (VAC- alpha ). BMS147 is suitable for ELISA application, Western blot analysis and is suitable as primary antibody for staining in FACS analysis. BMS147FI can be used for direct staining of cells. ... Applications Tested: Flow Cytometry (Intracellular Staining).
1044240
While both nitrocellulose and PVDF membranes are used for Western blotting and amino acid analysis, nitrocellulose is ideal in detecting low molecular weight proteins while PVDF is more suitable for detecting higher molecular weight proteins.
311372
['Laboratory for processing and cutting sections. ... ', 'Separate area for staining slides either manually or by machine.', 'Separate area for applying coverslips to slides.', 'Separate room or dedicated area with adequate ventilation/fume extraction for macroscopic examination of formalin-fixed specimens.']
649747
['Preparing the samples for running. ... ', 'An agarose TAE gel solution is prepared. ... ', 'Casting the gel. ... ', 'Setting up the electrophoresis chamber. ... ', 'Loading the gel. ... ', 'Electrophoresis. ... ', 'Stopping electrophoresis and visualizing the DNA.']
793040
Blotting paper referred to as bibulous paper is mainly used in microscopy to remove excess liquids from the slide before viewing. Blotting paper has also been sold as a cosmetic to aid in the removal of skin oils and makeup.
575009
For example, an antibody conjugated to HRP may be used to detect a small amount of a specific protein in a western blot. ... Horseradish peroxidase is also commonly used in techniques such as ELISA and Immunohistochemistry due to its monomeric nature and the ease with which it produces coloured products.
788853
['Titration (volumetric analysis)', 'Gravimetric analysis.', 'Various wet chemistry tests.', 'Combustion analysis.', 'Inert gas fusion.']
577114
['Antibody coating. Specific capture antibody is immobilized on high protein-binding plates by overnight incubation. ... ', 'Protein capture. Samples and standard dilutions are added to the wells and will be captured by the bound antibodies.', 'Detection antibody. ... ', 'Streptavidin-enzyme conjugate. ... ', 'Addition of substrate. ... ', 'Analysis.']
1469085
While RFLP produces bands, uses restriction enzymes, uses radioactive probes to visualize, and uses southern blotting, PCR and STR don't. PCR and STR uses PCR amplification prior to running a sample while RFLP does not.
1392006
['Fixation. Fixation is carried out immediately after the removal of the sample to be observed. ... ', 'Embedding. Embedding is the step that follows fixation in a fixative solution. ... ', 'Sectioning. Sectioning is performed using microtomy or cryotomy. ... ', 'Staining and immunolabeling. ... ', 'Mounting.']